家蚕小茧突变体sc生长发育相关通路差异表达基因转录分析  

Transcriptional analysis of differentially expressed genes of growth and development-related pathways in the small cocoon mutant sc of Bombyx mori

作　　者：吴赛 王闪闪 赵巧玲 朱娟[1,2] 王梅仙 唐顺明[1,2] 沈兴家 WU Sai;WANG Shan-Shan;ZHAO Qiao-Ling;ZHU Juan;WANG Mei-Xian;TANG Shun-Ming;SHEN Xing-Jia(Jiangsu Key Laboratory of Sericultural and Animal Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang 212100,China;Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture and Rural Affairs,Sericultural Scientific Research Center,Chinese Academy of Agricultural Sciences,Zhenjiang 212100,China)

机构地区：[1]江苏科技大学生物技术学院,江苏省蚕桑与畜禽生物技术重点实验室,镇江212100 [2]中国农业科学院桑蚕科学研究中心,农业农村部蚕桑遗传改良重点实验室,镇江212100

出　　处：《昆虫学报》2025年第1期1-13,共13页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(32072791);江苏省研究生科研创新项目(KYCX23_3916)。

摘　　要：【目的】航天蚕Bombyx mori后代小茧突变体sc的幼虫发育缓慢,食桑量少,推测其生长发育相关通路基因受到基因突变的影响。前期研究表明,sc突变受一对位于家蚕第3连锁群的隐性基因控制,但其控制基因并未被鉴定。本研究拟通过比较分析sc与航天蚕后代正常茧品系TG的转录组数据,为sc突变体相应基因的鉴定及分子机制的解析提供参考。【方法】分别取sc和TG 5龄第4天幼虫的头部和中肠组织进行转录组测序(RNA-seq),并通过比较转录组分析获得差异表达基因(differentially expressed genes,DEGs),对DEGs进行GO注释和KEGG富集分析。利用qRT-PCR验证随机选取的DEGs在sc和TG中的表达量。此外,利用qRT-PCR调查感兴趣基因在sc中的表达量。【结果】TG vs sc比较组头部检测到1528个DEGs,其中820个上调表达,708个下调表达;TG vs sc比较组中肠检测到1401个DEGs,其中683个上调表达,718个下调表达。GO分析表明头部和中肠DEGs在生物学过程中,大多数DEGs参与细胞过程、代谢过程、生物调节和刺激反应等;在分子功能中,大多数DEGs参与结合、催化活性、结构分子活性、转运蛋白活性和ATP依赖活性等。头部和中肠DEGs均涉及Hippo,Insulin和mTOR等与家蚕生长发育相关的信号通路。qRT-PCR分析显示,基因的表达趋势与转录组测序结果一致;与TG相比,sc中生长发育相关通路中BMSK0008105,BMSK0009907,BMSK0002689,BMSK0000286,BMSK0012340和BMSK00083629等关键基因差异表达。【结论】sc和TG中生长发育相关的信号通路关键基因的差异表达,通过影响生长发育过程中的能量代谢、器官发生和细胞生长、增殖及凋亡等生理过程,进而影响小茧突变体sc的体型发育。研究结果有助于阐明sc突变体形成的分子机制,并为家蚕体型调控研究积累实验数据。【Aim】The small cocoon mutant sc was discovered among the offspring of space silkworm(Bombyx mori),exhibiting slow larval development and reduced consumption of mulberry leaves.We speculate that genes related to growth and development pathways of the sc mutant may be affected by the gene mutation.Our previous research indicated that the sc mutant is controlled by a pair of recessive genes located on the 3rd linkage group of B.mori,but the responsible gene has not yet been identified.This study aims to provide insights into the identification of the responsible gene for the sc mutant and the analysis of its molecular mechanisms through comparative transcriptomic analysis between the sc mutant and the normal cocoon strain(TG)derived from space B.mori.【Methods】The head and midgut tissues from the day-45th instar larvae of sc and TG were collected for transcriptome sequencing(RNA-seq),respectively.The differentially expressed genes(DEGs)were obtained by comparative transcriptome analysis.Then GO annotation and KEGG enrichment analysis of DEGs were performed.qRT-PCR was employed to validate the expression levels of randomly selected DEGs in sc and TG and investigate the expression levels of the genes of interest in sc.【Results】A total of 1528 DEGs were detected in heads in the comparison group TG vs sc,with 820 DEGs showing up-regulated expression and 708 DEGs showing down-regulated expression.Similarly,1401 DEGs were identified in the midguts of the comparison group TG vs sc,with 683 DEGs showing up-regulated expression and 718 DEGs showing down-regulated expression.The GO analysis indicated that in biological processes,the majority of DEGs in the head and midgut were implicated in cellular process,metabolic process,biological regulation,response to stimulus,etc.In terms of molecular functions,most DEGs were associated with binding,catalytic activity,structural molecule activity,transporter activity and ATP-dependent activity.DEGs in the head and midgut were implicated in signaling pathways associated with

关 键 词：家蚕 航天蚕 小茧突变体 转录组测序 差异表达基因 功能注释 

分 类 号：S881.2[农业科学—特种经济动物饲养]