鼻咽癌患者COL1A1、MUC4表达与临床病理特征及预后的关系  

Relationship between COL1A1,MUC4 expression and clinicopathological features and prognosis in patients with nasopharyngeal carcinoma

作　　者：周卫 李汉琳 ZHOU Wei;LI Hanlin(Department of Otolaryngology,Jianghan University Affiliated Hospital,the Sixth Hospital of Wuhan,Wuhan,Hubei 430015,China;School of Medicine,Jianghan University,Wuhan,Hubei 430056,China)

机构地区：[1]江汉大学附属医院武汉市第六医院耳鼻喉科,湖北武汉430015 [2]江汉大学医学部,湖北武汉430056

出　　处：《国际检验医学杂志》2025年第4期391-397,共7页International Journal of Laboratory Medicine

基　　金：武汉市医学科研项目(WX20Z39)。

摘　　要：目的探讨鼻咽癌患者Ⅰ型胶原蛋白α1链(COL1A1)、黏蛋白4(MUC4)表达与临床病理特征及预后的关系。方法选取2020年12月至2021年6月该院收治的269例疑似鼻咽癌患者为研究对象,经过病理学检查确诊为鼻咽癌者123例作为研究组,其余经过病理学检查排除鼻咽癌的146例患者作为对照组。采用实时荧光定量聚合酶链反应检测COL1A1、MUC4 mRNA表达,免疫组织化学染色法测定COL1A1、MUC4蛋白表达。Kaplan-Meier法绘制生存曲线分析COL1A1、MUC4蛋白表达与鼻咽癌患者生存的关系。COX回归分析鼻咽癌患者预后不良的影响因素。绘制受试者工作特征(ROC)曲线分析COL1A1、MUC4 mRNA表达预测鼻咽癌患者预后不良的价值。结果与对照组比较,研究组COL1A1、MUC4 mRNA表达均升高,差异有统计学意义(P<0.05)。研究组COL1A1蛋白阳性表达率为58.54%(72/123)高于对照组[41.10%(60/146)],差异有统计学意义(P<0.05);MUC4蛋白阳性表达率为63.41%(78/123)高于对照组[33.56%(49/146)],差异有统计学意义(P<0.05)。研究组COL1A1、MUC4蛋白表达与TNM分期、肿瘤浸润深度、淋巴结转移有关(P<0.05)。与预后良好患者比较,预后不良患者COL1A1、MUC4 mRNA表达均升高,差异有统计学意义(P<0.05)。COL1A1蛋白阳性表达患者3年累积生存率显著低于COL1A1蛋白阴性表达患者,差异有统计学意义(P<0.05)。MUC4蛋白阳性表达患者3年累积生存率显著低于MUC4蛋白阴性表达患者,差异有统计学意义(P<0.05)。TNM分期Ⅲ/Ⅳ期、肿瘤浸润深度T3/T4期、有淋巴结转移、COL1A1、MUC4蛋白阳性表达为预后不良的危险因素(P<0.05)。ROC曲线分析结果显示,COL1A1、MUC4 mRNA联合预测鼻咽癌患者预后不良的曲线下面积(AUC)显著大于MUC4 mRNA单独预测的AUC,差异有统计学意义(Z=2.248,P=0.025)。结论鼻咽癌患者COL1A1、MUC4表达均升高,二者表达与鼻咽癌患者临床病理特征及预后密切相关,对鼻咽癌患者的预后Objective To investigate the relationship between collagen typeⅠalpha 1 chain(COL1A1)and mucin 4(MUC4)expression and clinicopathological features and prognosis in patients with nasopharyngeal carcinoma.Methods A total of 269 suspected patients with nasopharyngeal carcinoma admitted to a hospital from December 2020 to June 2021 were selected as the study subjects,123 patients with nasopharyngeal carcinoma confirmed by pathological examination were selected as the study group,and 146 patients with nasopharyngeal carcinoma ruled out by pathological examination were selected as the control group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the mRNA expression of COL1A1 and MUC4,and immunohistochemical staining was used to detect the protein expression of COL1A1 and MUC4.Survival curves were drawn by Kaplan-Meier method to analyze the relationship between COL1A1 and MUC4 protein expression and survival of nasopharyngeal carcinoma patients.COX regression analysis of nasopharyngeal carcinoma patients with adverse prognostic factors.Receiver operating characteristic(ROC)curve was drawn to analyze the value of COL1A1 and MUC4 mRNA expression in predicting poor prognosis of patients with nasopharyngeal carcinoma.Results Compared with the control group,the mRNA expressions of COL1A1 and MUC4 in the study group were increased,and the difference was statistically significant(P<0.05).The positive expression rate of COL1A1 protein in the study group was 58.54%(72/123)higher than that in the control group[41.10%(60/146)],and the difference was statistically significant(P<0.05).The positive expression rate of MUC4 protein was 63.41%(78/123)higher than that of control group[33.56%(49/146)],and the difference was statistically significant(P<0.05).The expressions of COL1A1 and MUC4 protein were correlated with TNM stage,depth of tumor invasion and lymph node metastasis(P<0.05).Compared with the patients with good prognosis,the expressions of COL1A1 and MUC4 mRNA were increased in the patients wi

关 键 词：鼻咽癌 Ⅰ型胶原蛋白α1链 黏蛋白4 预后 

分 类 号：R739.63[医药卫生—肿瘤]