淡绿杜洛埃藻(Drouetiella lurida)--分离自中国西藏的蓝藻新记录种  

Drouetiella lurida:a new record species of cyanobacteria isolated from Xizang,China

作　　者：王捷 张婷 蔡芳芳 董建新 李艳晖[1,2] 刘琪[4] Wang Jie;Zhang Ting;Cai Fangfang;Dong Jianxin;Li Yanhui;Liu Qi(College of Biological Science and Technology,Taiyuan Normal University,Jinzhong 030619,China;Shanxi Key Laboratory of Earth Surface Processes and Resource Ecology Security in Fenhe River Basin,Taiyuan Normal University,Jinzhong 030619,China;School of Animal Science and Nutritional Engineering,Wuhan Polytechnic University,Wuhan 430023,China;College of Life Science,Shanxi University,Taiyuan 030006,China)

机构地区：[1]太原师范学院生物科学与技术学院,山西晋中030619 [2]太原师范学院汾河流域地表过程与资源生态安全山西省重点实验室,山西晋中030619 [3]武汉轻工大学动物科学与营养工程学院,武汉430023 [4]山西大学生命科学学院,太原030006

出　　处：《河南师范大学学报(自然科学版)》2025年第2期63-72,共10页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(31970217;32000166);山西省应用基础研究计划(面上项目)(20210302124302);中央水生态环境保护专项项目(拉萨河流域水生态调查评估).

摘　　要：杜洛埃藻属(Drouetiella Mai,Johansen&Pietrasiak)中的物种具有形态可塑性,容易与细点丝藻科(Oculatellaceae)的其他物种混淆,该属中的藻株从陆地生物群落中分离,采用包括分子手段、形态特征和生态信息相结合的多相方法进行表征.从中国西藏自治区拉萨市的潮湿土壤分离出一株丝状蓝藻,编号为SXACC0052,形态上与淡绿杜洛埃藻(Drouetiella lurida(Gomont)Mai,Johansen&Pietrasiak)相似,藻丝都呈现橄榄绿色,有无色且坚固的鞘,横壁不收缩或稍微收缩,没有观察到假分枝.16S rRNA基因与16S-23S rRNA ITS序列显示出这株蓝藻与淡绿杜洛埃藻有较高的相似度,并且在系统发育树中聚为一支,步展值分别为98/78/1和85/99/0.89.此外,这株丝状蓝藻的ITS二级结构与淡绿杜洛埃藻的结构相似度较高,进一步表明这株丝状蓝藻属于淡绿杜洛埃藻.杜洛埃藻属是我国首次报道的新记录属,淡绿杜洛埃藻是我国首次报道的新记录种.该种的发现丰富了我国蓝藻物种多样性,为保护和持续了解藻种资源提供理论依据.Species in the genus Drouetiella Mai,Johansen&Pietrasiak are morphologically plastic,easily confused with other species in the family of Oculatellaceae,in which strains are isolated from terrestrial communities and characterized using a polyphasic approach that includes molecular,morphological and ecological information.A filamentous cyanobacteria was isolated from the moist soil of Lhasa,Xizang Autonomous Region,China,named SXACC0052.The morphology of isolated strain was similar to Drouetiella lurida((Gomont)Mai,Johansen&Pietrasiak),the trichomes were olive-green,with colorless and firm sheaths,the cross-walls were not constricted or slightly contracted,no false-branching was observed.The comparative analysis of 16S rRNA gene and 16S-23S rRNA ITS sequences showed that the strain shared high similarities with Drouetiella lurida and clustered into a single branch in the phylogenetic tree with bootstrap value of 98/78/1 and 85/99/0.89,respectively.In addition,the ITS secondary structure of this filamentous cyanobacteria has a high similarity to that of Drouetiella lurida,which further indicated that this filamentous cyanobacteria belonged to Drouetiella lurida.Drouetiella is a new record genus reported for the first time in China,Drouetiella lurida is a new record species reported for the first time in China.The discovery of this species enriches the species diversity of cyanobacteria in China and provides a theoretical basis for the conservation and continuous understanding of algae resources.

关 键 词：淡绿杜洛埃藻 西藏 蓝藻 新记录 系统发育 

分 类 号：Q949.22[生物学—植物学]