机构地区:[1]遵义医科大学第三附属医院,遵义市第一人民医院检验科,贵州遵义563000 [2]遵义医科大学第三附属医院,遵义市第一人民医院中心实验室,贵州遵义563000
出 处:《遵义医科大学学报》2025年第2期111-119,共9页Journal of Zunyi Medical University
基 金:贵州省2021年省级重点建设学科项目(临床检验诊断学);2024年度贵州省基础研究计划自然科学项目[NO:黔科合基础-ZK(2024)一般675];贵州省高层次创新型百层次人才[NO:黔科合平台人才-GCC(2022)042-1]。
摘 要:目的明确长链非编码RNA LINC01123在宫颈鳞癌中的表达情况,并探讨其对宫颈鳞癌细胞增殖、迁移的作用及其潜在机制。方法通过GEPIA数据库分析LINC01123在宫颈鳞癌中的表达情况,收集了27例宫颈癌组织及癌旁组织标本,通过Arraystar LncRNA表达谱芯片技术分析其中3例样本差异表达的lncRNA,并通过RT-qPCR法验证宫颈癌组织、宫颈鳞癌细胞SiHa和C33a中LINC01123的表达情况。通过慢病毒和腺病毒分别构建敲低和过表达LINC01123的SiHa细胞株,采用CCK8法和划痕实验检测其对SiHa细胞增殖、迁移能力的作用,并通过RT-qPCR和Western blot检测敲低LINC01123对SiHa细胞中SLC7A11和GPX4的调控作用。结果Arraystar LncRNA芯片分析发现宫颈鳞癌组织中存在5760个上调和6975个下调的lncRNA,其中LINC01123的表达水平显著高于癌旁组织(P<0.05)。经RT-qPCR进一步验证,LINC01123在宫颈鳞癌组织及宫颈鳞癌细胞系中均高表达,并以SiHa细胞高表达最为显著(P<0.05)。敲低SiHa细胞中的LINC01123水平,其增殖和迁移能力受到显著抑制(P<0.05),反之,SiHa细胞的增殖能力显著增强(P<0.01)。此外,敲低LINC01123还抑制了SiHa细胞中SLC7A11和GPX4的mRNA和蛋白质的表达(P<0.05)。结论敲低LINC01123可抑制宫颈鳞癌细胞的增殖与迁移,其机制可能涉及对铁死亡关键蛋白SLC7A11和GPX4的表达调控。Objective To elucidate the expression of LncRNA LINC01123 in cervical squamous cell carcinoma and investigate its impact on the proliferation and migration of cervical cancer cells,along with its underlying mechanism.Methods The expression and prognosis of LINC01123 in cervical squamous cell carcinoma were analyzed by GEPIA database.Samples of 27 cervical cancer tissues and adjacent tissues were collected.LncRNA differentially expressed in 3 samples were analyzed by Arraystar LncRNA expression profiling technology.The expression of LINC01123 in cervical cancer tissue,cervical squamous cell SiHa and C33a was verified by RT-qPCR.Knockdown and overexpression models of LINC01123 were established in SiHa cells using lentivirus and adenovirus,respectively,with knockdown and overexpression efficiency verified by RT-qPCR.The impact of LINC01123 on the proliferation and migration of SiHa cells was evaluated using CCK8 and scratch assays.Furthermore,western blot and RT-qPCR were employed to investigate the influence of LINC01123 knockdown on the expression and transcription levels of SLC7A11 and GPX4 proteins in SiHa cells.Results A total of 5760 upregulated and 6975 down-regulated lncRNAs were identified through microarray sequencing.In cancerous tissues,the expression of LINC01123 was significantly higher compared to adjacent tissues(P<0.05).RT-qPCR analysis revealed high expression of LINC01123 in cervical cancer tissues and cell lines SiHa and C33a,particularly in SiHa(P<0.05).Functional assays revealed that reducing LINC01123 levels inhibited proliferation and migration of SiHa cells(P<0.05),while overexpression hindered its proliferation(P<0.01).Knockdown of LINC01123 was found to suppress the transcription and expression of SLC7A11 and GPX4 as confirmed by RT-qPCR and Western blot analysis.Conclusion Knockdown of LINC01123 inhibits the proliferation and migration of cervical squamous carcinoma cells by a mechanism that may involve the regulation of the expression of the key iron-death proteins SLC7A11 and GPX4.
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