伴发桥本甲状腺炎的甲状腺良恶性结节患者血清外泌体miRNA的差异表达分析  

Differential expression analysis of serum exosomal miRNA in benign and malignant thyroid nodules with Hashimotos thyroiditis

作　　者：乔文靖 魏相然 尹峰燕 张辉[4] 曹文源 罗晓婷 何倩 邢浩 QIAO Wenjing;WEI Xiangran;YIN Fengyan;ZHANG Hui;CAO Wenyuan;LUO Xiaoting;HE Qian;XING Hao(Hematology Center,Qingdao University Affiliated Tai'an Central Hospital,Shandong Tai'an 271000,China;School of Public Health and Health Management,Shandong First Medical University,Shandong Jinan 250000,China;Department of Thyroid Surgery,Qingdao University Affiliated Tai'an Central Hospital,Shandong Tai'an 271000,China;Department of Laboratory Medicine,Qingdao University Affiliated Tai'an Central Hospital,Shandong Tai'an 271000,China)

机构地区：[1]青岛大学附属泰安市中心医院血液病诊疗中心,山东泰安271000 [2]山东第一医科大学公共卫生与健康管理学院,山东济南250000 [3]青岛大学附属泰安市中心医院甲状腺外科,山东泰安271000 [4]青岛大学附属泰安市中心医院检验医学科,山东泰安271000

出　　处：《现代肿瘤医学》2025年第3期396-402,共7页Journal of Modern Oncology

基　　金：山东省自然科学基金(编号:ZR2023QH135);山东省泰安市科技创新课题(编号:2022NS234)。

摘　　要：目的:分析伴发与不伴发桥本甲状腺炎(Hashimotos thyroiditis,HT)情况下,甲状腺乳头状癌(papillary thyroid carcinoma,PTC)与良性甲状腺结节患者血清外泌体微小RNA(microRNA,miRNA)表达谱,为临床上甲状腺结节良恶性的鉴别提供参考依据。方法:选取伴发桥本甲状腺炎的PTC患者、单纯PTC患者、伴发桥本甲状腺炎的甲状腺结节患者、单纯甲状腺结节患者血清样本,提取外泌体miRNA进行高通量测序,预测差异表达miRNA及靶基因并进行GO富集分析和KEGG富集分析。结果:在伴发桥本甲状腺炎组,PTC和良性组相比,24个血清外泌体miRNA表达水平存在明显差异。在不伴发桥本甲状腺炎组,PTC与良性组相比,11个血清外泌体miRNA表达水平存在明显差异。结合两组,有1个共同差异表达miRNA,即miR-147b-3p,均表达为上调。KEGG分析结果表明,miR-147b-3p富集丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)或磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)信号通路,均与PTC发生有关。结论:有无伴发桥本甲状腺炎情况下,PTC和良性结节组血清外泌体miRNA表达谱存在差异,miR-147b-3p可用于甲状腺结节良恶性的诊断。Objective:To analyze the serum exosomal microRNA(miRNA) expression profiles of patients with papillary thyroid carcinoma(PTC) and benign thyroid nodules with and without Hashimotos thyroiditis(HT),and to provide a reference basis for the differentiation of the benign and malignant nature of thyroid nodules in clinical practice.Methods:Serum samples from patients with concomitant Hashimoto PTC,simple PTC,with Hashimoto's thyroid nodules,and simple thyroid nodules were selected,and exosomal miRNAs were extracted and subjected to highthroughput sequencing for the prediction of differentially expressed miRNAs and target genes,and then subjected to GO enrichment analysis and KEGG enrichment analysis.Results:In the concomitant Hashimoto group,there was a significant difference in the expression levels of 24 serum exosomal miRNAs between the PTC and compared with the benign group.In the unaccompanied Hashimoto group,there was a significant difference in the expression levels of 11in the PTC and compared with the benign group.Combining the two groups,there was 1 common differentially expressed miRNA,miR-147b-3p,both of which were expressed in an unregulated manner.KEGG analysis showed that miR-147b-3p was enriched for either mitogen-activated protein kinase(MAPK) or phosphatidylinositol 3-kinase(PI3K) signalling pathways,both of which are implicated in the development of PTC.Conclusion:With or without concomitant Hashimoto condition,there were differences in serum exosomal miRNA expression profiles between PTC and benign nodule groups.miR-147b-3p can be used for the diagnosis of benign and malignant thyroid nodules in both cases.

关 键 词：甲状腺乳头状癌 桥本甲状腺炎 外泌体 microRNA 

分 类 号：R736.1[医药卫生—肿瘤]