miR-574-3p、FAM3C在食管癌组织中的表达及其与患者临床病理特征的关系  

Expression of miR-574-3p and FAM3C in esophageal cancer tissue and their relationship with clinicopathological characteristics of patients

作　　者：杨志强 常国涛 史文松 马晓斌 钱河 YANG Zhiqiang;CHANG Guotao;SHI Wensong;MA Xiaobin;QIAN He(Department of Thoracic Surgery,the Fifth Clinical Medical College of Henan University of Chinese Medicine(Zhengzhou People's Hospital),Henan Zhengzhou 450053,China)

机构地区：[1]河南中医药大学第五临床医学院(郑州人民医院)胸外科,河南郑州450053

出　　处：《现代肿瘤医学》2025年第3期427-432,共6页Journal of Modern Oncology

摘　　要：目的:探究微小RNA(miR)-574-3p、序列相似家族3成员C(family with sequence similarity 3 member C,FAM3C)在食管癌组织中的表达,并分析二者与患者临床病理特征的关系。方法:选取2018年5月至2020年5月于本院行手术治疗的90例食管癌患者,收集术中留取的癌组织和癌旁组织标本。使用qRT-PCR法检测组织中miR-574-3p、FAM3C mRNA相对表达量;使用免疫组织化学法检测组织中FAM3C蛋白表达情况;采用Spearman等级相关分析食管癌组织中miR-574-3p、FAM3C的相关性;Kaplan-Meier法分析miR-574-3p、FAM3C与预后的关系。结果:食管癌患者癌组织中miR-574-3p低表达,FAM3C mRNA、FAM3C蛋白高表达(P<0.05)。食管癌组织中miR-574-3p、FAM3C蛋白表达水平具有负相关性(r=-0.420,P<0.05)。Pearson相关性分析显示,食管癌组织中miR-574-3p、FAM3C mRNA表达具有负相关性(r=-0.731,P<0.05)。miR-574-3p、FAM3C蛋白均与TNM分期、浸润程度、淋巴结转移有关(P<0.05)。miR-574-3p高表达组3年累积生存率为83.72%,显著高于miR-574-3p低表达组(53.19%);FAM3C高表达组3年累积生存率为56.86%,显著低于FAM3C低表达组(82.05%),差异均有统计学意义(log rankχ^(2)=10.175、7.290,P<0.05)。结论:食管癌组织中miR-574-3p表达水平较低,FAM3C表达水平较高,且二者具有负相关性,与临床病理特征中的TNM分期、浸润程度、淋巴结转移以及患者3年累积生存率有关。Objective:To investigate the expression of microRNA(miR)-574-3p and family with sequence similarity 3 member C(FAM3C) in esophageal cancer tissue and their relationship with clinicopathological characteristics in esophageal cancer patients.Methods:From May 2018 to May 2020,90 patients with esophageal cancer who underwent surgical treatment in our hospital were collected,and specimens of cancer tissue and adjacent tissue were collected during the surgery.The qRT-PCR method was applied to detect the relative expression levels of miR-574-3p and FAM3C mRNA in tissues.Immunohistochemical method was applied to detect the expression of FAM3C.protein in tissues.Spearman rank correlation was applied to analyze the correlation.The relationship between the expression levels of miR-574-3p and FAM3C proteins and the clinical pathological characteristics of patients was analyzed.Kaplan-Meier method was applied to analyze the relationship between miR-574-3p and FAM3C protein expression levels and prognosis.Results:Low expression of miR-574-3p and high expression of FAM3C mRNA and FAM3C protein in cancer tissues of patients with esophageal cancer(P <0.05).There was a negative correlation between miR-574-3p and FAM3C(r=-0.420,P <0.05).Pearson correlation analysis showed that the mRNA expressions of miR-574-3p and FAM3C in esophageal cancer tissues were negatively correlated(r=-0.731,P <0.05).miR-574-3p and FAM3C proteins were correlated with TNM stage,invasion degree and lymph node metastasis(P <0.05).The 3-year cumulative survival rate of the miR-574-3p high expression group was 83.72%,which was obviously higher than that of the miR-574-3p low expression group(53.19%).The 3-year cumulative survival rate of the FAM3C high expression group was 56.86%,obviously lower than that of the FAM3C low expression group(82.05%)(log rank χ^(2)=10.175,7.290,P <0.05).Conclusion:The expression level of miR-574-3p is low in esophageal cancer tissue,while the expression level of FAM3C is high,and there is a negative correlation between the them,w

关 键 词：食管癌 微小RNA-574-3p 序列相似家族3成员C 临床病理特征 

分 类 号：R735.1[医药卫生—肿瘤]