基于转录组分析色膜覆盖下月月竹的竹青变色机制  

The discoloration mechanism of Chimonobambusa sichuanensis clum under colored film covering based on transcriptome analysis

作　　者：刘昌来[1] 熊星[1] 李天帅 段跃华 杨峰 刘国华[1] LIU Changlai;XIONG Xing;LI Tianshuai;DUAN Yuehua;YANG Feng;LIU Guohua(Co-Innovation Center for Sustainable Forestry in Southern China,Bamboo Research Institute,Nanjing Forestry University,Nanjing 210037,China;Heze Chemical Technician College,Shandong 274500,Chin)

机构地区：[1]南京林业大学南方现代林业协同创新中心/竹类研究所,南京210037 [2]菏泽化工技师学院,山东菏泽274500

出　　处：《植物生理学报》2025年第1期83-96,共14页Plant Physiology Journal

基　　金：宜宾智慧林业重大项目(YBZD2024-2-2);国家自然科学基金(31870575)。

摘　　要：为了明确月月竹(Chimonobambusa sichuanensis)竹青变色的条件和分子机制,本研究以寒竹属竹种月月竹为研究对象,对其进行无色膜和橙色膜覆盖处理。竹青处理50 d后,观察竹青的颜色,测定薄膜透光性,同时测定竹青中的花青素含量并进行转录组分析。结果表明:月月竹竹青经50 d处理,无色膜覆盖下竹青呈现紫色,而橙色膜覆盖仍然为绿色;两种膜透光性测定发现,无色膜透过蓝、绿和红光,而橙膜无法透过蓝光,只有透过红光和绿光;色素含量测定发现两种膜覆盖后的竹青中总叶绿素含量差异不显著,但无色膜处理下花青素和类胡萝卜素含量显著高于橙膜处理;对两种膜处理的竹青进行转录组测序,共获得50.51 Gb测序数据,进行差异基因分析。无色膜相对于橙色膜处理,共获得1 298个差异表达基因,其中580个显著上调表达, 718个显著下调表达。差异基因GO富集分析表明,差异基因明显富集到了类囊体膜、质体膜等光合系统结构的GO terms (Gene Ontology terms);KEGG富集显示差异基因主要富集到淀粉和蔗糖代谢、苯丙烷生物合成、光合作用、碳固定、类黄酮生物合成等代谢途径中,富集到光合作用和光合系统中的基因在无色膜处理下明显上调表达。进一步分析发现17个明显富集到花青素合成和类黄酮代谢中的基因在无色膜处理下上调表达,而在橙膜下明显下调表达。通过荧光定量PCR对上调表达基因进行验证发现编码4CL、CAD、CCR、CYP75B1、DFR、POD、BGL、PAL等蛋白的13个基因在无色膜处理下的竹青中明显上调表达,而在橙膜处理下的竹青中明显下调表达。本次研究结果表明蓝光是诱导月月竹竹青变色的主要环境因子,能显著提高月月竹竹青中类胡萝卜素和花青素含量,转录组结果数据表明,蓝光通过增强苯丙烷代谢途径促进了花青素和木质素等黄酮类物质合成。To clarify the conditions and molecular mechanisms of the discoloration of the bamboo shoot epidermis of Chimonobambusa sichuanensis.In this study,Chimonobambusa sichuanensis,a bamboo spe-cies of the genus Chimonobambusa,was selected as the research object and subjected to treatments of colorless film and orange film coverage.After 50 days of treatment on the bamboo clum,the color of the bamboo shoot epidermis was observed,the light transmittance of the flms was determined,the anthocy-anin content in the bamboo bark was measured,and transcriptome analysis was conducted.The results showed that after 50 days of treatment on the bamboo clum of C.sichuanensis,the bamboo bark under the colorless film coverage turned purple,while that under the orange film coverage remained green.The determination of the light transmittance of the two films revealed that the colorless film transmitted blue,green,and red light,while the orange film did not transmit blue light and only transmitted red and green light.The determination of pigment content found that there was no significant difference in the total chlo-rophyll content in the bamboo bark under the coverage of the two films,but the anthocyanin and carot-enoid contents under the colorless film treatment were significantly higher than those under the orange film treatment.Transcriptome sequencing was performed on the bamboo shoot epidermis treated with the two films,and a total of 50.51 Gb sequencing data was obtained.Through different expression genes analysis,compared with the orange film treatment,a total of 1298 different expression genes were ob-tained under the colorless film treatment,among which 580 genes were significant up-regulated expres-sion and 718 were significant down-regulated expression.GO enrichment analysis of the different expres-sion genes indicated that the different expression genes were significantly enriched in the Go terms of photosynthetic system structures such as thylakoid membranes and plastid membranes.KEGG enrich-ment showed that the different

关 键 词：月月竹 竹秆变色 蓝光 花青素 转录组 

分 类 号：S687[农业科学—观赏园艺]