基于5-HT/TRPV4/AQP5探讨抑郁相关干眼的发病机制  

Mechanism of depression-related dry eye based on 5-HT/TRPV4/AQP5

作　　者：杨颖 张嘉之 姬慧杰 丁园晨 高卫萍 Yang Ying;Zhang Jiazhi;Ji Huijie;Ding Yuanchen;Gao Weiping(Department of Ophthalmology,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu Province,China)

机构地区：[1]南京中医药大学附属医院眼科,中国江苏省南京市210029

出　　处：《国际眼科杂志》2025年第3期372-377,共6页International Eye Science

基　　金：江苏省中医药科技发展计划项目(No.QN202315);江苏省中医院院级科研基金(No.Y22056)。

摘　　要：目的:基于5-HT/TRPV4/AQP5探讨抑郁相关干眼大鼠泪液分泌减少的机制。方法:健康雄性SD大鼠用慢性不可预见性温和刺激(CUMS)构造抑郁模型后,筛选出抑郁相关干眼大鼠8只,空白组为空白大鼠8只,ELISA法比较两组大鼠血清和海马组织5-HT,观察泪腺HE切片和AQP5免疫组化,并通过Western blot和RT-PCR检测两组泪腺中5-HT3R、TRPV4和AQP5表达。结果:抑郁相关干眼组泪液分泌明显减少(P=0.001),血清和海马5-HT水平明显下降(均P<0.05),泪腺AQP5抗体免疫组化阳性率明显低于空白组(P<0.001),同时泪腺中5-HT3R、TRPV4和AQP5蛋白和基因表达明显下降(均P<0.05),并且泪腺组织切片未见明显炎性细胞。结论:抑郁相关干眼的发生可能与非炎性的5-HT/TRPV4/AQP5机制有关。AIM:To investigate the mechanism of reduced tear secretion in depression-related dry eye rats based on 5-HT/TRPV4/AQP5.METHODS:Healthy SD male rats were established with chronic unpredictable mild stress(CUMS)method to establish a depression-induced dry eye model(n=8),and the control group was blank rats(n=8).The ELISA method was used to compare the 5-hydroxytryptamine(5-HT)in serum and hippocampal tissue of the two groups,and the HE sections of lacrimal gland and AQP5 immunohistochemistry were observed.Western blot and RT-PCR were used to detect the expression of 5-HT3R,TRPV4 and AQP5 in the lacrimal gland tissue of the two groups of rats.RESULTS:The tear secretion in the depression-induced group was significantly reduced(P=0.001),the serum and hippocampal 5-HT levels were significantly reduced(all P<0.05),the expression of AQP5 antibody in the lacrimal gland immunohistochemistry was significantly lower than that in the control group(P<0.001),the expression of 5-HT3R,TRPV4 and AQP5 in the lacrimal gland was significantly reduced(all P<0.05),and no obvious inflammatory cells were found in the lacrimal gland tissue sections.CONCLUSION:Depression-related dry eye may occur through a non-inflammatory 5-HT/TRPV4/AQP5 mechanism.

关 键 词：抑郁相关干眼 泪液分泌 5-HT/TRPV4/AQP5 

分 类 号：R73[医药卫生—肿瘤]