Anti-metastatic potential of OR3 pigment from Streptomyces coelicolor JUACT03 on MDA-MB-231 breast cancer in vitro and in vivo  

作　　者：Somasekhara Derangula Varalakshmi K Nadumane 

机构地区：[1]Department of Biotechnology,School of Sciences,JAIN(Deemed-to-be-University),Bangalore,Karnataka,India [2]Center for Research in Pure and Applied Sciences,JAIN(Deemed-to-be-University),Bangalore 560078,Karnataka,India

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2025年第2期43-52,I0006-I0008,共13页亚太热带生物医学杂志(英文版)

摘　　要：Objective:To investigate the mechanism of anticancer activity of a pigment OR3 from Streptomyces coelicolor in in vitro and in vivo metastatic breast cancer models and to characterize the pigment.Methods:The anticancer mechanism was analyzed in MDA-MB-231 cells using MTT,lactate dehydrogenase,caspase,DNA fragmentation,clonogenic,flow cytometry,Western blot,and scratch assays.The effects of OR3 on xenograft mouse models were evaluated by tumor volume measurement,hematological analysis,and histopathological observation.The characterization of OR3 was also performed using gas chromatograohy-mass spectrometry and nuclear magnetic resonance spectroscopy.Results:OR3 exhibited potent cytotoxicity against MDA-MB-231 cells,with no observed effects on HEK-293 cells.Caspase-9 activation was detected in OR3-treated MDA-MB-231 cells.Flow cytometry showed a dose-dependent induction of apoptosis and cell cycle arrest at the sub-G_(1) and S phases.Furthermore,OR3 completely inhibited MDA-MB-231 cell migration and demonstrated anti-proliferative effects by downregulating the protein expression of KPNA2,XPO1,RAB5B,and p38 MAPK.In in vivo studies,OR3 was non-toxic to mice,inhibited tumor xenograft growth,and maintained normal hematological parameters and tissue architecture.Nuclear magnetic resonance spectroscopy demonstrated the presence of a prodigiosin-like compound,while gas chromatography-mass spectrometry analysis identified additional compounds in OR3.Conclusions:Our findings validate OR3 pigment as a promising compound for the treatment of metastatic breast cancer,warranting further studies.

关 键 词：Breast cancer MDA-MB-231 Tumor growth inhibition APOPTOSIS OR3 pigment Streptomyces coelicolor METASTASIS 

分 类 号：R285[医药卫生—中药学]