Ligustroside derived from Ligustrum japonicum inhibits melanogenesis via blocking the MAPK and PKA/CREB signaling pathways  

作　　者：Xian-Rong Zhou Fatih Karadeniz Jung Hwan Oh Youngwan Seo Chang-Suk Kong 

机构地区：[1]Department of Bioscience,Silla University,Busan 46958,Korea [2]Collaborative Innovation Center for Child Nutrition and Health Development,Chongqing University of Education,Chongqing 400067,China [3]Marine Biotechnology Center for Pharmaceuticals and Foods,Silla University,Busan 46958,Korea [4]Nutritional Education,Graduate School of Education,Silla University,Busan 46958,Korea [5]Division of Convergence on Marine Science,Korea Maritime&Ocean University,Busan,49112,Korea [6]Department of Food and Nutrition,Silla University,Busan 46958,Korea

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2025年第2期65-74,I0004,I0005,共12页亚太热带生物医学杂志(英文版)

基　　金：supported by the BB21plus funded by Busan Metropolitan City and Busan Techno Park,and the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.NRF-2023R1A2C1006268 and RS-2023-00212560).

摘　　要：Objective:To investigate the anti-melanogenic potential of ligustroside isolated from Ligustrum japonicum.Methods:The cytotoxicity of ligustroside was tested via MTT assay.Furthermore,the effects of ligustroside on the expression of critical melanogenic markers such as tyrosinase,tyrosinase related proteins(TRPs),and microphthalmia-associated transcription factor(MITF)were analyzed at both mRNA and protein levels via RT-qPCR and Western blot,respectively,inα-melanocyte stimulating hormoneinduced B16F10 cells.In addition,phosphorylation of p38,ERK and JNK proteins was investigated.Immunofluorescence analysis of MITF was also conducted.Results:Ligustroside significantly reduced intracellular tyrosinase activity and melanin content by 37.11%and 29.12%,respectively,compared to untreated cells.Moreover,it downregulated the expression of MITF,tyrosinase,TRP-1,and TRP-2 at the mRNA and protein levels by regulating both the mitogen-activated protein kinase(MAPK)and protein kinase A(PKA)/cAMP response element-binding protein(CREB)signaling pathways.Ligustroside also suppressed the nuclear protein expression of MITF,β-catenin,and p-CREB,and decreased immunofluorescence intensity of nuclear MITF.Conclusions:Ligustroside derived from Ligustrum japonicum shows a significant anti-melanogenesis effect via suppression of the MAPK and PKA/CREB signaling pathways.

关 键 词：Ligustrum japonicum Ligustroside MAPK MELANOGENESIS CREB PKA 

分 类 号：R73[医药卫生—肿瘤]