Effects of thinning and ground cover plants on soil bacterial community composition and diversity in Picea asperata plantations within giant panda habitats  

疏伐和地被植物对大熊猫栖息地云杉人工林土壤细菌群落组成和多样性的影响

作　　者：Xiang Tao Qiuju Xiao Huanhuan Shao Weiguo Tu Ling Li Yuanbin Zhang Hao Jiang 

机构地区：[1]Institute of Mountain Hazards and Environment,Chinese Academy of Sciences,Chengdu 610299,China [2]College of Life Science,Sichuan Normal University,Chengdu 610101,China [3]Sichuan Provincial Academy of Nature Resource Sciences,Chengdu 610015,China

出　　处：《Journal of Plant Ecology》2024年第6期93-104,共12页植物生态学报(英文版)

基　　金：supported by the Sichuan Science and Technology Program,China(Grant No.2023YFS0372).

摘　　要：Forest thinning and ground cover plant management play crucial roles in habitat enhancement,yet their effects on soil microbiota remain poorly understood.This study examines their impact on soil properties and bacterial communities in artifcial spruce forests(Picea asperata)within China’s Huangtuliang ecological corridor,a crucial habitat for giant pandas.Thinning signifcantly alters soil pH and total phosphorus(TP)levels,with minimal changes observed in total nitrogen(TN),microbial biomass carbon(MBC)and nitrogen(MBN).The combined effect of thinning and ground cover presence increases soil organic carbon(SOC)to 65.47 g/kg,contrasting with its absence.Thinning enhances the abundance of Proteobacteria,Acidobacteria and Chlorofexi while reducing Actinobacteria.Conversely,ground cover removal decreases Proteobacteria and Bacteroidetes but increases Chlorofexi,Verrucomicrobia and Rokubacteria.These changes lead to reduced bacterial community diversity,as indicated by a lower Shannon diversity index and distinct community composition differences demonstrated through beta-diversity analysis.Soil pH,TP and MBN are crucial in maintaining bacterial community structure,with pH and TP exhibiting the strongest correlations.Network analysis confrms the signifcant infuence of TP and pH on bacterial genera across various phyla.This study reveals the role of stochastic processes in highelevation,low-temperature ecological corridors(R^(2)=0.817),with thinning’s impact varying depending on the ground cover presence,thus enhancing effects post-removal by reducing dispersal limitation(migration rate,m=0.96).These fndings highlight the ecological implications of habitat management in sensitive ecosystems and advance our understanding of microbial dynamics in critical habitats.森林疏伐和地被植物管理在改善栖息地方面至关重要,但其对土壤微生物群落的影响仍然知之甚少。本研究揭示了森林疏伐和地被植物对大熊猫重要栖息地--中国黄土梁生态廊道内云杉人工林(Picea asperata)土壤性质和细菌群落的影响。研究结果显示,疏伐显著改变了土壤的pH值和总磷水平,对总氮、微生物生物量碳氮的影响很小。疏伐和地被植物覆盖的综合效应使土壤有机碳含量增加到65.47 g/kg,与没有地被植物覆盖时形成鲜明对比。疏伐增加了变形菌、酸杆菌和绿弯菌的丰度,同时减少了放线菌。相反,去除地被植物会减少变形菌门和拟杆菌门,但会增加绿弯菌门和疣微菌门。这些变化导致细菌群落多样性降低,即香农多样性指数降低、群落组成存在明显差异。土壤pH值、总磷和微生物生物量氮对维持细菌群落结构至关重要,其中pH值和总磷的相关性最强。网络分析证实了总磷和pH值对不同门细菌属的显著影响。本研究揭示了随机过程在高海拔、低温生态廊道中的作用(R^(2)=0.817),疏伐的影响随地被植物的存在而变化,通过减少扩散限制(迁移率=0.96)来增强去除后的效果。上述发现强调了敏感生态系统中栖息地管理的生态意义,并提升了学术界对关键栖息地中微生物动态的认识。

关 键 词：BAMBOO ecological corridors microbial composition soil properties THINNING understory removal 

分 类 号：S15[农业科学—土壤学]