Kinesin family member 14 expression and its clinical implications in colorectal cancer  

作　　者：Kai Qin Jia-Yuan Luo Da-Tong Zeng Wan-Ying Huang Bin Li Qi Li Yan-Ting Zhan Rong-Quan He Wei-Jian Huang Gang Chen Zu-Yuan Chen Bang-Teng Chi Yu-Xing Tang Rui-Xue Tang Hui Li 

机构地区：[1]Department of Pathology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China [2]Department of Pathology,Redcross Hospital of Yulin City,Yulin 537000,Guangxi Zhuang Autonomous Region,China [3]Department of Medical Oncology,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China [4]Department of Pathology,The First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,Jinan 250000,Shandong Province,China [5]Department of Colorectal and Anal Surgery,The First Affiliated Hospital of Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China

出　　处：《World Journal of Gastrointestinal Oncology》2025年第3期299-321,共23页世界胃肠肿瘤学杂志(英文)

基　　金：Natural Science Foundation of Shandong Province,No.ZR2020QH185;Scientific Research Nurturing Fund of The First Affiliated Hospital of Shandong First Medical University&Shandong Provincial Qianfoshan Hospital,No.QYPY2020NSFC0803;Guangxi Zhuang Autonomous Region Health Commission Scientific Research Project,No.Z-A20220415;Guangxi Medical University Teacher Teaching Ability Development Project,No.2022JFA02;Guangxi Medical University Undergraduate Education and Teaching Reform Project,No.2023Y05.

摘　　要：BACKGROUND Colorectal cancer(CRC)is the third most common cancer globally,causing over 900000 deaths annually.Risk factors include aging,diet,obesity,sedentary lifestyle,tobacco use,genetic predisposition,and inflammatory bowel disease.Despite current treatments,survival rates for advanced CRC remain low,highlighting the need for better therapeutic strategies.AIM To evaluate both the clinical significance and the pathological implications of the Kinesin family member 14(KIF14)expression within CRC specimens.Additionally,this study aims to investigate the interaction between nitidine chloride(NC)and KIF14,considering their potential as therapeutic targets.METHODS The expression of the KIF14 protein in CRC was analyzed using immunohistochemical staining.The integration of multicenter high-throughput data facilitated the calculation of the standardized mean difference(SMD)for KIF14 mRNA levels.The assessment of clinical and pathological impact was enhanced by analyzing combined receiver operating characteristic curves,along with measures of sensitivity,specificity,and likelihood ratios.Additionally,clustered regularly interspaced short palindromic repeats knockout screening for cell growth and single-cell sequencing were employed to validate the significance of KIF14 expression in CRC.Survival analysis established the prognostic value of KIF14 in CRC.The molecular mechanism of NC against CRC was elucidated through whole-genome sequencing and enrichment analysis,and molecular docking was utilized to explore the targeting affinity between NC and KIF14.RESULTS KIF14 was highly expressed in 208 CRC patients.Data from 17 platforms involving 2436 CRC samples and 1320 noncancerous colorectal tissue controls indicated that KIF14 expression was significantly higher in CRC samples,with an SMD of 1.92(95%CI:1.49-2.35).The area under the curve was 0.94(95%CI:0.92-0.96),with a sensitivity of 0.85(95%CI:0.78-0.90)and a specificity of 0.90(95%CI:0.85-0.93).The positive and negative likelihood ratios were 8.38(95%CI:5.39-13.02)and

关 键 词：Colorectal cancer Kinesin family member 14 Nitidine chloride Molecular docking Standardized mean difference 

分 类 号：R735.34[医药卫生—肿瘤]