Assessment of the biosafety profile of Galium verum in vitro on myoblasts and in ovo on chorioallantoic membrane  

作　　者：Diana Maria Morariu-Briciu Sorin Lucian Bolintineanu Andreea Rata Alexandra Denisa Semenescu Alina Anton Robert Jijie Andreea Kis Ingrid Hrubaru Alina Heghes 

机构地区：[1]Department of Anatomy and Embryology,“Victor Babes”University of Medicine and Pharmacy,Timisoara 300041,Romania [2]Doctoral School,“Victor Babes”University of Medicine and Pharmacy,Timisoara 300041,Romania [3]Department of Vascular Surgery,“Victor Babes”University of Medicine and Pharmacy,Timisoara 300041,Romania [4]Department of Toxicology,Drug Industry,Management and Legislation,“Victor Babes”University of Medicine and Pharmacy,Timisoara 300041,Romania [5]Research Center for Pharmaco-Toxicological Evaluations,“Victor Babes”University of Medicine and Pharmacy,Timisoara 300041,Romania [6]Department of Obstetrics and Gynecology,“Victor Babes”University of Medicine and Pharmacy,Timisoara 300041,Romania [7]Department of Pharmaceutical Technology,“Victor Babes”University of Medicine and Pharmacy,Timisoara 300041,Romania

出　　处：《World Journal of Cardiology》2025年第2期71-84,共14页世界心脏病学杂志(英文)

摘　　要：BACKGROUND Cardiovascular diseases are the first cause of death in the world.Ischemic heart disease is the main cause of heart failure.New approaches are continuously sou-ght to identify better therapeutic success.Thereby,current research has been drawn to identifying and completing the therapeutic profile of natural sources.Galium species are representatives exhibiting diuretic and antibacterial potential in living organisms and can treat burns,wounds,and skin diseases.Moreover,it was also observed that these plants manifest cardioprotective effects as well as having antihemolytic,antioxidant,antibacterial,anti-inflammatory,immunomod-ulatory,and antiproliferative potential.In ischemic heart disease,Galium verum(G.verum)extract manifested preservative properties in terms of contractility,systolic and diastolic function maintenance,and reduced damage to the heart after ischemia.In addition,G.verum extract upregulated the activity of antioxidant enzymes alleviating the production of pro-oxidants.AIM To test the ethanolic extract of G.verum on the H9C2(2-1)cell line by evaluating the in vitro biosafety profile and in ovo irritative potential.METHODS Cells were tested in vitro for viability(using the MTT test),cellular morphology,cell number,confluence,nuclear morphology(by immunofluorescence staining of cell nuclei and F-actin assay)and in ovo by the hen’s egg chorioal-lantoic membrane(CAM)test and CAM anti-irritant methods to study the irritation potential on the CAM.RESULTS The extract demonstrated a dose-dependent stimulatory activity.The viability increased to 170%for the dose of 55µg/mL and decreased to 135%at 200µg/mL.The results of cell number,confluence,and morphological analysis did not present significant changes compared with control untreated cells.The immunofluorescence assay showed insignificant apoptotic potential,and the hen’s egg CAM test revealed that the extract was in the weak to mode-rately irritating category with an irritation score of 5.3.When applying the sample to the CAM,only slight

关 键 词：Galium verum L Ethanolic extract CARDIOMYOCYTES Ischemic heard Cellular viability stimulation In vitro In ovo 

分 类 号：R28[医药卫生—中药学]