基于转录组挖掘叶用莴苣中花青素生物合成代谢关键基因  

Identification of key genes involved in anthocyanin biosynthesis in Lactuca sativa L.based on transcriptome analysis

作　　者：姜永强 王丽慧[1,2] 孙雪梅 JIANG Yongqiang;WANG Lihui;SUN Xuemei(Academy of Agriculture and Forestry Sciences,Qinghai University,Xining 810016,China;Qinghai Key Laboratory of Vegetable Genetics and Physiology,Xining 810016,China)

机构地区：[1]青海大学农林科学院,青海西宁810016 [2]青海省蔬菜遗传与生理重点实验室,青海西宁810016

出　　处：《江苏农业学报》2025年第1期150-160,共11页Jiangsu Journal of Agricultural Sciences

基　　金：青海省科学技术厅重点研发与转化计划项目(2024-NK-106)。

摘　　要：为探究调控叶用莴苣叶片花青素合成的主效基因,本研究对叶用莴苣大速生(绿色)和紫霞(紫色)的转录组数据进行分析。结果表明,大速生和紫霞比较组中共检测到7 232个差异表达基因,其中相对表达量上升基因4 214个,相对表达量下降基因3 018个。KEGG数据库富集分析结果表明,富集到代谢过程中的差异表达基因最多,花青素生物合成和次生代谢产物-其他抗生素生物合成通路中差异表达基因富集程度较高。在大速生和紫霞比较组中共鉴定到55个转录因子家族,差异表达基因大多数属于MYB、bHLH、C_(2)C_(2)和AP2/ERF转录因子家族基因。从MYB转录因子基因中筛选出log_(2)FC绝对值较大的LOC111917799,命名为LsMYB1基因。瞬时表达试验结果验证了LsMYB1基因可以调控花青素的生物合成。本研究结果为叶用莴苣叶色的分子遗传机理及进一步的基因功能研究奠定了基础。In order to explore the major effective genes regulating anthocyanin synthesis in Lactuca sativa L.,this study analyzed the transcriptome data of Dasusheng(green) and Zixia(purple).The results showed that a total of 7 232 differentially expressed genes were detected in the comparison group of Dasusheng and Zixia,including 4 214 genes with increased relative expression and 3 018 genes with decreased relative expression.The results of KEGG database enrichment analysis indicated that the most differentially expressed genes were enriched in the metabolic process,and the enrichment degrees of the differentially expressed genes in anthocyanin biosynthesis and secondary metabolite-other antibiotic biosynthesis pathways were relatively high.A total of 55 transcription factor families were identified in the comparison group of Dasusheng and Zixia.Most of the differentially expressed genes belonged to the MYB,bHLH,C_(2)C_(2) and AP2/ERF transcription factor family genes.LOC111917799 with a larger absolute value of log_(2FC) was screened out from the MYB transcription factor genes and named as LsMYB1.The transient expression experiment verified that the LsMYB1 gene could regulate the biosynthesis of anthocyanins.The results of this study lay a foundation for the molecular genetic mechanism of leaf color in leaf lettuce and further research on gene function.

关 键 词：叶用莴苣 花青素 转录组 差异表达基因 

分 类 号：S636.2[农业科学—蔬菜学]