基于非靶向代谢组学的绿豆芽代谢物差异分析  

Differential analysis of metabolites in mung bean sprouts based on non-targeted metabolomics

作　　者：王新妤 张鸣慧 姚玲 张丁 张振鑫 申慧芳[1] 郭锋[2] WANG Xinyu;ZHANG Minghui;YAO Ling;ZHANG Ding;ZHANG Zhenxin;SHEN Huifang;GUO Feng(Department of Basic Sciences,Shanxi Agricultural University,Taigu 030801,China;College of Resources and Environment,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学基础部,山西太谷030801 [2]山西农业大学资源与环境学院,山西太谷030801

出　　处：《江苏农业学报》2025年第1期175-183,共9页Jiangsu Journal of Agricultural Sciences

基　　金：山西省重点研发计划项目(201803D221020-3)。

摘　　要：为了比较不同品种绿豆芽的代谢物差异,利用非靶向代谢组学分析方法对晋绿豆2号豆芽和滩绿116豆芽的代谢物进行分析。结果表明,从2种豆芽中共检测出1 968种差异代谢物。根据重要性投影值(VIP)≥1和P<0.05的标准,共筛选得到差异显著的代谢物1 064种,占总差异代谢物的54.07%。这1 064种差异代谢物主要分布在丙烷、哌啶和吡啶类生物碱生物合成通路,氨基糖和核苷酸糖代谢通路、膦酸盐和次膦酸盐代谢通路,萜类主干生物合成通路,糖酵解/糖异生通路中。差异代谢物中,葫芦巴碱具有降血糖的作用,其代谢途径为烟酸和烟酰胺代谢途径,该代谢途径属于丙烷、哌啶和吡啶类生物碱生物合成通路,滩绿116豆芽中葫芦巴碱含量显著高于晋绿豆2号豆芽。本研究结果为绿豆芽用种质资源挖掘提供了理论依据。In order to compare the metabolic differences of different varieties of mung bean sprouts,non-targeted metabolomics analysis was used to analyze the metabolites of Jinlü No.2 and Tanlü No.116.The results showed that 1 968 differential metabolites were detected in the two kinds of bean sprouts.According to the standard of variable importance in the projection(VIP)≥1 and P<0.05,a total of 1 064 significantly different metabolites were screened out,accounting for 54.07% of the total differential metabolites.These 1 064 differential metabolites were mainly distributed in the biosynthesis pathways of propane,piperidine and pyridine alkaloids,the metabolic pathways of aminosugars and nucleotide sugars,the metabolic pathways of phosphonates and phosphinates,the biosynthesis pathways of terpenoids,and the glycolysis/gluconeogenesis pathways.Among the differential metabolites,trigonelline had the hypoglycemic effect,its metabolic pathway was the nicotinic acid and nicotinamide metabolic pathway.This metabolic pathway belonged to propane,piperidine,and pyridine alkaloids.And the content of trigonelline in Tanlü No.116 bean sprouts was significantly higher than that in Jinlü No.2 bean sprouts.The results of this study provide a theoretical basis for the exploration of germplasm resources for mung bean sprouts.

关 键 词：绿豆芽 非靶向代谢组学 差异代谢物 代谢途径 

分 类 号：S522[农业科学—作物学]