茶树BZR1基因家族的鉴定及CsBZR1-5响应干旱胁迫的分子机理研究  

Cloning of BZR1 Gene Family in Tea Plants and Molecular Mechanism Study of CsBZR1-5 Response to Drought Stress

作　　者：董圆 张永恒 肖烨子 余有本[3] DONG Yuan;ZHANG Yongheng;XIAO Yezi;YU Youben(Yangling Vocational&Technical College,Yangling 712100,China;Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008,China;Northwest A&F University,Yangling 712100,China)

机构地区：[1]杨凌职业技术学院,陕西杨凌712100 [2]中国农业科学院茶叶研究所,浙江杭州310008 [3]西北农林科技大学,陕西杨凌712100

出　　处：《茶叶科学》2025年第1期15-28,共14页Journal of Tea Science

基　　金：国家现代农业产业技术体系(CARS-19)。

摘　　要：BZR1转录因子是油菜素内酯(Brassinosteroid,BR)信号转导途径中的关键转录因子,在植物生长发育以及胁迫响应过程中发挥重要的调节作用。基于茶树基因组数据,鉴定并克隆了6个茶树BZR1家族成员,分析了它们的基因结构、编码蛋白的亚细胞定位和转录激活活性,并探究了它们在不同组织和干旱胁迫下的表达模式。结果表明,6个茶树BZR1成员的内含子个数为2或3,其编码蛋白都包含典型的bHLH特征结构域;亚细胞定位结果显示,除了CsBZR1-1定位于细胞质和细胞核,其余CsBZR1s均定位于细胞核;转录激活活性分析表明,CsBZR1s在酵母中均具有转录激活活性;不同组织中的表达模式分析显示,CsBZR1s在茶树不同组织中的表达具有特异性,其中CsBZR1-1和CsBZR1-6的表达模式较为相似;干旱胁迫下的表达模式分析表明,6个CsBZR1基因均响应干旱胁迫,其中CsBZR1-5的表达持续被PEG模拟的干旱胁迫诱导。此外,ABA合成途径中的关键酶基因CsNCED1在干旱胁迫下与CsBZR1-5的表达模式高度相似,凝胶电泳迁移试验(Electrophoretic mobility shift azssay,EMSA)分析发现,CsBZR1-5能够与CsNCED1启动子上的E-box元件结合,说明CsBZR1-5可能参与调控了CsNCED1对干旱胁迫的响应过程。本研究系统分析了6个CsBZR1的基本特征和功能,为进一步阐明CsBZR1成员在茶树生长发育和干旱胁迫响应中的调控作用奠定了基础。The BZR1 transcription factor is a key transcription factor in the brassinosteroid(BR)signaling pathway,playing an important regulatory role in plant growth,development,and stress response.This study identified and cloned six members of the BZR1 family in tea plants based on genomic data.Their gene structures,subcellular localization of encoded proteins,and transcriptional activation activities were analyzed,and their expression patterns under different tissues and drought stress were explored.The results show that the number of introns in the 6 BZR1 members of tea plants was 2 or 3,and their encoded proteins all contained typical bHLH characteristic structural domains.Subcellular localization analysis shows that except for CsBZR1-1,which was localized in the cytoplasm and nucleus,all other CsBZR1s were localized in the nucleus.Transcriptional activation activity analysis shows that CsBZR1s exhibited transcriptional activation activity in yeast.The analysis of expression patterns in different tissues shows that CsBZR1s had specificity in expression in different tissues of tea plants,among which the expression patterns of CsBZR1-1 and CsBZR1-6 were relatively similar.The expression pattern analysis under drought stress shows that all six CsBZR1 genes were responsive to drought stress.The expression of CsBZR1-5 was continuously induced by drought stress simulated by PEG.In addition,the expression pattern of the key enzyme gene CsNCED1 in ABA synthesis pathway was highly similar to that of CsBZR1-5 under drought stress.The analysis of Electrophoretic Mobility Shift Assay(EMSA)found that CsBZR1-5 can bind to the E-box element on the CsNCED1 promoter,indicating that CsBZR1-5 may be involved in regulating the response of CsNCED1 to drought stress.This study systematically analyzed the basic characteristics and functions of six CsBZR1 members,laying the foundation for further elucidating the regulatory roles of CsBZR1 members in tea plant growth and development and drought stress response.

关 键 词：茶树 CsBZR1s基因 亚细胞定位 转录自激活 凝胶电泳迁移试验 

分 类 号：S571.1[农业科学—茶叶生产加工] Q946[农业科学—作物学]