茶树DREB基因的生信分析及其调控CsPOD3的抗旱机制  

Bioinformatic Analysis of DREB Genes and Regulation of CsPOD3 on Drought Tolerance Mechanisms in Camellia sinensis

作　　者：徐容 邓智英 邵陈禹 罗玉麒 仇舒琪 王聪 周玲红 刘仲华[1] 沈程文[1] XU Rong;DENG Zhiying;SHAO Chenyu;LUO Yuqi;QIU Shuqi;WANG Cong;ZHOU Linghong;LIU Zhonghua;SHEN Chengwen(Key Laboratory of Tea Science of Ministry of Education,Hunan Agricultural University,Changsha 410128,China;Chenzhou Institute of Agricultural Sciences,Chenzhou 423042,China)

机构地区：[1]湖南农业大学茶学教育部重点实验室,湖南长沙410128 [2]郴州市农业科学研究所,湖南郴州423042

出　　处：《茶叶科学》2025年第1期29-42,共14页Journal of Tea Science

基　　金：国家自然科学基金(32372765);国家重点研发计划(2022YFD1600801);湖南省现代农业产业技术体系(HARS-10);湖南省自然科学区域联合基金(2023JJ50061)。

摘　　要：基于课题组前期的转录组数据筛选到CsDREB11、CsDREB15和CsDREB25等基因。通过RT-qPCR验证,它们均受干旱诱导表达。从进化树、蛋白理化性质、亲/疏水性、二级和三级结构、亚细胞定位等方面进行预测与分析。结果表明,CsDREB11、CsDREB15和CsDREB25都与狭叶油茶(Camellialanceoleosa)的亲缘性最为密切,都属于不稳定的亲水性蛋白。二级/三级结构均以无规则卷曲和α-螺旋为主,其亚细胞定位均位于细胞核中。课题组前期的研究发现CsDREB25与CsPOD3均在干旱下高表达且呈显著相关,推测CsDREB25和CsPOD3存在相互作用关系。通过酵母单杂交验证(Y1H)和双荧光素酶报告基因检测(DLA)以及双荧光素酶互补成像(LCI)等方法试验验证了CsDREB25能正向调控CsPOD3的表达,提高POD的活性,清除过多活性氧,提高茶树的耐旱性。Based on the previous transcriptome data of the research group,three genes,CsDREB11,CsDREB15,and CsDREB25,were screened.Verified by RT-qPCR,their expressions were induced by drought.The phylogenetic tree,physical and chemical properties,hydrophilicity/hydrophobicity,secondary and tertiary structures,and subcellular localization were predicted and analyzed.The results show that CsDREB11,CsDREB15,and CsDREB25 are most closely related to their homolog genes in Camellia lanceoleosa.They are all unstable hydrophilic proteins.Random coils andα-helices dominate the secondary/tertiary structures.Their subcellular localizations are located in the nucleus.The preliminary study of the subject found that both CsDREB25 and CsPOD3 were highly expressed and significantly correlated under drought conditions,suggesting that there was an interaction between them.Through Y1H,DLA,and LCI experiments,it was verified that CsDREB25 can positively regulate the expression of CsPOD3,increase the activity of POD,remove excessive reactive oxygen species,and improve the drought tolerance of tea plants.

关 键 词：茶树 干旱胁迫 DREBs 过氧化物酶 生物信息学分析 

分 类 号：S571.1[农业科学—茶叶生产加工] Q946[农业科学—作物学]