茶树ICE基因家族鉴定及CsICE43克隆和低温表达分析  

Identification of Tea ICE Gene Family and Cloning and Expression Analysis of CsICE43 under Low-temperature

作　　者：朱倩[1] 邵陈禹 周彪 刘硕谦[1] 刘仲华[1] 田娜[1] ZHU Qian;SHAO Chenyu;ZHOU Biao;LIU Shuoqian;LIU Zhonghua;TIAN Na(National Engineering Research Center for the Utilization of Plant Functional Components,Key Laboratory of Tea Science,Ministry of Education,Hunan Agricultural University,Changsha 410128,China)

机构地区：[1]国家植物功能成分利用工程技术研究中心,湖南农业大学茶学教育部重点实验室,湖南长沙410128

出　　处：《茶叶科学》2025年第1期43-60,共18页Journal of Tea Science

基　　金：国家重点研发计划项目(2021YFD1200203);国家自然科学基金联合基金重点项目(U22A20500);湖南省现代农业产业技术体系项目(HARS-10);国家茶树育种联合攻关项目(GJCSYZLHGG-12)。

摘　　要：近年来全球极端低温天气频发,严重影响了茶树的产量和品质。ICE(Inducer of CBF expression)基因家族主要参与植物的低温胁迫响应,但在茶树领域中的相关研究还不够全面。本研究从茶树基因组中鉴定出51个茶树CsICEs基因,对其理化性质、基因结构和启动子顺式作用元件展开生物信息学分析。茶树CsICEs基因的启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件,其可能参与多种逆境胁迫响应。转录组分析和RT-qPCR验证结果发现,低温下CsICE43基因的表达量上升了4.24倍,其可能与茶树低温响应相关。以茶树品种‘保靖黄金茶1号’的cDNA为模板,克隆获得了CsICE43基因,其在不同组织中的表达模式存在差异,在顶芽和嫩叶中特异性高表达。蛋白氨基酸序列和系统进化树分析表明,CsICE43基因包含与ICE家族其他成员一致的S-rich、bHLH、ACT等保守结构域,且与毛花猕猴桃(Actinidiaeriantha)的亲缘关系较近。在STRING在线网站中以拟南芥AtICEs为模型,推测茶树CsICE43蛋白与HOS1、MYB15、DREB1/2存在潜在的互作关系。亚细胞定位试验表明CsICE43定位于细胞核,与跨膜结构分析结果一致。综上所述,本研究发现CsICE43基因可能与茶树低温响应关联,为深入挖掘其基因功能与抗寒分子机理提供了一定的理论基础。In recent years,extreme low-temperature weather has frequently occurred worldwide,significantly affecting the yield and quality of tea plants.The ICE(Inducer of CBF expression)gene family plays a crucial role in the low-temperature stress response of plants.However,research specifically focused on tea plants is still limited.This study identified 51 ICE genes from the tea genome and performed a bioinformatics analysis to examine their physical and chemical properties,gene structure,and promoter cis-acting elements.The promoter regions of the CsICE genes are rich in cis-acting elements related to light response,plant hormones,growth and development,and abiotic stress,suggesting their involvement in various stress responses.Transcriptome analysis and RT-qPCR verification indicate that the expression of the CsICE43 increased 4.24 folds under low-temperature conditions,highlighting its potential role in the low-temperature response of tea plants.To further investigate this,the cDNA of tea cultivar‘Baojing Golden Tea No.1’was used as a template to clone the CsICE43 gene.Its expression varied across tissues,with exceptionally high levels observed in terminal buds and young leaves.Further amino acid sequence and phylogenetic tree analysis indicate that the CsICE43 gene contains conserved domains such as S-rich,bHLH,and ACT,which are consistent with other members of the ICE family.It is closely related to Actinidia eriantha.The STRING online database utilized Arabidopsis thaliana AtICEs to hypothesize potential interactions between CsICE proteins and HOS1,MYB15,and DREB1/2.Subcellular localization experiments demonstrate that CsICE43 is located in the nucleus,which is consistent with the findings from the transmembrane structure analysis.In summary,this study suggests that the CsICE43 gene may be associated with the low-temperature response in tea plants,providing a theoretical foundation for further exploration of its gene function and the molecular mechanisms underlying cold resistance.

关 键 词：茶树 ICE基因家族 抗寒 生物信息学 表达分析 

分 类 号：S571.1[农业科学—茶叶生产加工] S435.711[农业科学—作物学]