多黏类芽孢杆菌抗真菌活性及其纳米硒化应用  

Study on Antifungal Activity and Nano-selenization Application of Paenilbacillus polymyxa

作　　者：袁莲莲[1] 刘天波 李晓东 邓征宇 滕凯 申莉莉[1] 焦裕冰 王英文 杨金广[1] 李莹 YUAN Lianlian;LIU Tianbo;LI Xiaodong;DENG Zhengyu;TENG Kai;SHEN Lili;JIAO Yubing;WANG Yingwen;YANG Jinguang;LI Ying(Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao 266101,China;Hunan Branch of China National Tobacco Corporation,Changsha 410004,China;Qingdao Agricultural Technology Promotion Center,Qingdao 266071,China)

机构地区：[1]中国农业科学院烟草研究所,山东青岛266101 [2]中国烟草总公司湖南省公司,湖南长沙410004 [3]青岛农业技术推广中心,山东青岛266071

出　　处：《河南农业科学》2025年第1期101-108,共8页Journal of Henan Agricultural Sciences

基　　金：中国烟草总公司绿色防控重大专项(110202201020(LS-04));青岛市科技惠民示范专项(23-2-8-xdny-12-nsh);中国农科院创新工程所级重点项目(ASTIP-TRIC-ZD04)。

摘　　要：为筛选防效优良的微生物应用于作物真菌类病害防治,对前期筛选得到的多黏类芽孢杆菌KZJ-1进行了抗病及纳米硒化研究。结果显示,KZJ-1菌液对烟草炭疽病菌、靶斑病菌、根腐病菌、黑胫病菌均具有较好的抑制效果,抑制率分别为49.78%、53.64%、46.42%、58.85%。菌株KZJ-1可以生物合成纳米硒,不同浓度的纳米硒活性菌液对黑胫病菌均具有较好的抑制效果,随着加入的亚硒酸钠浓度增大,抑制效果增强。同时,盆栽试验结果显示,KZJ-1菌液及其纳米硒活性菌液均可有效防治烟草黑胫病并促进烟株生长,灌施纳米硒活性菌液7 d后烟草黑胫病病情指数最低,防效达90.23%,14 d后防效稍有下降,仍高达83.32%,均显著高于药剂对照;纳米硒活性菌液灌根的本氏烟生长发育最好,株高、最大叶长、最大叶宽的增长率分别为16.21%、31.74%、33.61%。可见,菌株KZJ-1生物合成纳米硒增强了原始菌株的抗病及促生能力,在生产上具有一定的实际应用价值。In order to screen the microorganisms with good control effect for the control of crop fungal diseases,the disease resistance and nano-selenization of Paenobacillus polymyxa KZJ-1 were studied.The results showed that KZJ-1 bacteriological solution had good inhibition effect on Colletotrichum micotianae,Thanatephorus cucumeris,Thielaviopsis basicola and Phytophthora parasitica,with inhibition rates of 49.78%,53.64%,46.42%and 58.85%,respectively.In addition,the strain KZJ-1 could biosynthesize nano-selenium,and the active solution of nano-selenium with different concentrations had a good inhibitory effect on Phytophthora parasitica,and the inhibitory effect was enhanced with the increase of the concentration of sodium selenite added.At the same time,the pot experiment results showed that KZJ-1 and its nano-selenium active bacterial solution could effectively prevent and control tobacco black shank disease and promote the growth of tobacco plants.The disease index of tobacco black shank disease treated with nano-selenium active bacterial solution was the lowest after 7 days of irrigation,and the control effect reached 90.23%,and the control effect decreased slightly to 83.32%after 14 days,both of which were significantly higher than that of the control.The growth rate of plant height,maximum leaf length and maximum leaf width were 16.21%,31.74%and 33.61%,respectively.It can be seen that the biosynthesis of nano-selenium by strain KZJ-1 enhanced the disease resistance and growth promotion ability of the original strain,and had certain practical application value in production.

关 键 词：多黏类芽孢杆菌 烟草病害 真菌 病害防治 纳米硒 促生 

分 类 号：S476[农业科学—农业昆虫与害虫防治] S435.72[农业科学—植物保护]