不同方式有氧运动介导乙酰化修饰对小鼠肾肿瘤的干预研究  

The Study on Intervention of Acetylated Modification Mediated by Different Forms of Aerobic Exercise on Renal Tumor in Mice

作　　者：毛海峰 陈嘉勤[3] 周雯艳[2] 朱敬生[3] 陈薇薇[3] 李文敏[3] 周柏存[3] MAO Haifeng;CHEN Jiaqin;ZHOU Wenyan;ZHU Jingsheng;CHEN Weiwei;LI Wenmin;ZHOU Bocun(Department of Physical Education of Suqian University,Suqian 223800,China;Department of Physical Education of Yichun University,Yichun 336000,China;Department of Physical Education of Hunan Normal University,Changsha 410012,China)

机构地区：[1]宿迁学院体育学院,宿迁223800 [2]宜春学院体育学院,宜春336000 [3]湖南师范大学体育学院,长沙410012

出　　处：《中国细胞生物学学报》2025年第2期185-194,共10页Chinese Journal of Cell Biology

基　　金：江西省教育厅科学技术研究项目(批准号:GJJ201626);江西省卫生健康委科技计划(批准号:202110128);宿迁学院人才引进科研启动基金(批准号:校2022XRC056)资助的课题。

摘　　要：该文探讨不同方式有氧运动通过介导乙酰化修饰对小鼠肾肿瘤的干预效果及机制。对40只健康雄性昆明(KM)小鼠进行癌细胞侵袭肾包膜模型构建,模型构建成功后将其随机分为模型对照组(M)、跑台组(TM)、无负重游泳组(SW)、负重游泳组(HL)等4组,另取10只健康雄性KM小鼠作为空白组(B)不进行癌细胞侵袭。将3个运动组按照运动方案进行有氧运动,运动结束后剥离出肿瘤组织,称重,计算肿瘤抑制率;采用HE染色法光镜下对肿瘤及肾组织进行显微结构形态学观察;免疫组织化学染色法检测P300、Tip60蛋白表达情况;qRT-PCR法检测HDAC2、HDAC3、HDAC8m RNA表达情况。结果显示, 3个运动组肿瘤的生长均受到了抑制,以无负重游泳组抑制率最高,其次为跑台组和负重游泳组;肾组织HE染色中模型对照组肾间质损伤程度最高, 3个运动组的肾间质损伤程度均出现不同程度降低,肿瘤组织HE染色中模型对照组肿瘤细胞受损程度最低, 3个运动组的肿瘤细胞受损程度均出现不同程度升高;P300蛋白表达量以负重游泳组为最高,并按模型对照组、无负重游泳组、跑台组、空白组依次降低。Tip60蛋白表达量以负重游泳组为最低,并按模型对照组、无负重游泳组、跑台组、空白组依次升高;HDAC2 m RNA的表达量以空白组最低,并按跑台组、无负重游泳组、模型对照组、负重游泳组依次增高;HDAC3 m RNA的表达情况与HDAC2相似,但负重游泳组的表达量低于模型对照组;各组HDAC8的表达量高低趋势同HDAC3。总之,中小强度跑台或无负重游泳运动对肿瘤的发生发展具有一定的抑制作用,可通过组蛋白乙酰基转移酶及组蛋白去乙酰化酶来调控组蛋白的乙酰化水平,进而影响肿瘤细胞的生成及抑制。This study aimed to explore the intervention effect and possible mechanism by different forms of aerobic exercise mediating acetylation modification on mouse kidney tumors. Forty healthy male KM mice were used to construct the model of cancer cell invasion into renal capsule. Then, they were randomly divided into four groups: model control group(M), treadmill group(TM), swimming group(SW), heavy load swimming group(HL),and another 10 healthy male KM mice were taken as blank group(B) without cancer cell invasion. According to the exercise program, three exercise groups took aerobic exercise. After the end of the exercise, tumor tissue were taken out, weighted, and the tumor inhibition rate was calculated. Tumor and renal tissue's microscopic morphology was observed under the light microscope by HE staining;expression of P300 and Tip60 proteins were detected by immunohistochemical staining;HDAC2, HDAC3 and HDAC8 mRNA expression was detected by qRT-PCR. The results showed that the tumor inhibition rate of group SW was the highest, followed by the group TM and the group HL;the degree of renal interstitial injury of group M was the highest, and the renal interstitial injury of three exercise groups were decreased in different degree;the tumor cells damage of group M was the lowest, and the tumor cells damage of three exercise groups were increased in different degrees;the P300 protein expression of group HL was the highest, followed by group M, group SW, group TM, group B;the Tip60 protein expression of group HL was the lowest, followed by group M, group SW, group TM, group B;the HDAC2 mRNA expression of group B was the lowest, followed by group TM, group SW, group M, group HL;the HDAC3 mRNA expression was similar to HDAC2, but the expression of group HL was lower than that of group M;the HDAC8 mRNA expression was the same as HDAC3. In conclusion, medium and small intensity treadmill or no load swimming exercise has a certain inhibitory effect on the tumor. The acetylation level of histones can be regulated by histone

关 键 词：有氧运动 肾肿瘤 乙酰化 组蛋白乙酰转移酶 组蛋白去乙酰化酶 

分 类 号：R737.11[医药卫生—肿瘤]