复方心衰康胶囊对心衰大鼠SERCA2a表达的调控作用  

The Regulatory Effect of Compound Xinshuaikang Capsule on the Expression of SERCA2a in Rats with Heart Failure

作　　者：杨皎 张晶 YANG Jiao;ZHANG Jing(Gardiology Department,Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine,Inner Mongolia,Hohhot 010010,China)

机构地区：[1]内蒙古自治区中医医院心病科,内蒙古呼和浩特010010

出　　处：《光明中医》2025年第2期287-290,共4页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：内蒙古自然科学基金资助项目(No.2020MS08160)。

摘　　要：目的观察复方心衰康胶囊对心衰大鼠SERCA2a表达以及心功能的影响。方法选取SPF级雄性SD大鼠120只,分为空白组(20只)和模型组(100只),利用阿霉素腹腔注射构建大鼠心衰模型,将成功建模后的大鼠(91只)随机分为模型组(19只)、地高辛组(18只)和复方心衰康低剂量组(18只)、中剂量组(18只)、高剂量组(18只)。分别检测各实验组心功能指标、SERCA2a及其mRNA基因表达水平。结果与空白组比较,模型组、地高辛组、复方心衰康低中高剂量组大鼠的LVEDD、LVFS、NT-proBNP、miR-25-3p表达水平显著升高,而SERCA2a及其mRNA表达水平、LVEF均明显降低(P<0.05);与模型组比较,复方心衰康低中高剂量组和地高辛组LVEDD、LVFS、NT-proBNP和miR-25-3p表达的水平显著降低,SERCA2a及其mRNA表达水平、LVEF均显著升高(P<0.05)。结论复方心衰康胶囊可能通过抑制miR-25-3p调控SERCA2a相关蛋白表达改善心衰大鼠的心功能。Objective To observe the effect of compound Xinshuaikang capsule on the expression of SERCA2a and cardiac function in rats with heart failure.Methods A total of 120 male SD rats with SPF grade were selected and divided into blank group(n=20)and model group(n=100).The rat heart failure model was constructed by adriamycin intraperitoneal injection.After successful modeling,the rats(n=91)were randomly divided into model group(n=19),digoxin group(n=18),compound Xinshuaikang low-dose group(n=18),medium-dose group(n=18)and high-dose group(n=18).Cardiac function indexes,SERCA2a and its mRNA gene expression levels were detected in each experimental group.Results Compared with blank group,the expression levels of LVEDD,LVFS,NT-proBNP and miR-25 were significantly increased in model group,digoxin group and Compound Xinshuaikang low-middle-high dose group,while the expression levels of SERCA2a,its mRNA and LVEF were significantly decreased(P<0.05).Compared with model group,the expression levels of LVEDD,LVFS,NT-proBNP and miR-25 were significantly decreased in compound Xinshuaikang low-medium-high-dose group and digoxin group,while SERCA2a and its mRNA expression levels and LVEF were significantly increased(P<0.05).Conclusion Compound Xinshuaikang capsule can improve the cardiac function of CHF rats by inhibiting the expression of SERCA2a related protein by inhibiting miR-25.

关 键 词：心衰 复方心衰康胶囊 SERCA2A miR-25-3p 实验研究 

分 类 号：R54[医药卫生—心血管疾病]