香藿喷雾剂对小鼠上呼吸道感染的防治作用研究  

A study on the preventive and therapeutic effects of Xiang Huo Spray on upper respiratory tract infections in mice

作　　者：李婷 颜宏 龚吕东 钟承志 林瑞超 朱高倩[1,2] 孙文强 吴德松 郭琰 LI Ting;YAN Hong;GONG Lv-dong;ZHONG Cheng-zhi;LIN Rui-chao;ZHU Gao-qian;SUN Wen-qiang;WU De-song;GUO Yan(Yunnan Institute of Materia Medica,Kunming 650111,China;Yunnan Province Company Key Laboratory for TCM and Ethnic Drug of New Drug Creation,Kunming 650111,China;Beijing University of Chinese Medicine,Beijing 100029,China)

机构地区：[1]云南省药物研究所,昆明650111 [2]云南省中药和民族药新药创制企业重点实验室,昆明650111 [3]北京中医药大学,北京100029

出　　处：《中国新药杂志》2025年第4期407-413,共7页Chinese Journal of New Drugs

基　　金：云南省特色植物筛选与研发服务CXO平台建设资助项目(2022YKZY001)。

摘　　要：目的:考察香藿喷雾剂对小鼠上呼吸道感染的防治作用。方法:采用小鼠浓氨水引咳模型、气管酚红排泌模型和二甲苯诱导的小鼠急性鼻炎模型评价香藿喷雾剂的止咳化痰作用和对急性鼻炎的改善作用。通过脂多糖(lipopolysaccharide,LPS)诱导小鼠巨噬细胞促炎介质释放,并利用纸片扩散法和二倍浓度稀释法检测香藿喷雾剂的体外抗炎作用和对呼吸道主要致病菌的体外抑菌作用。结果:香藿喷雾剂在明显降低小鼠的咳嗽次数的同时升高气管酚红排泌量,明显改善小鼠急性鼻炎的病理改变,降低炎症和出血。香藿喷雾剂体外明显抑制细胞促炎介质的释放,对呼吸道主要致病菌具有明显体外抑菌活性,表现出良好的体外抗炎作用和抗菌作用。结论:香藿喷雾剂具有止咳化痰、抗炎、抗菌的药理作用,能明显改善小鼠急性鼻炎的病理改变,减少炎症和出血,对上呼吸道感染具有一定防治作用。Objective:To investigate the prevention and treatment effect of Xiang Huo Spray(XHS)on upper respiratory tract infection in mice.Methods:The anticough and expectorant effects of XHS and its effect for improvement of acute rhinitis were evaluated by using concentrated ammonia water cough model,phenol red excretion model and acute rhinitis model induced by xylene in mice.The release of pro-inflammatory mediators from mouse macrophages induced by LPS,disk diffusion and double dilution method were used to detect the anti-inflammatory and antibacterial effect of XHS on major respiratory pathogens in vitro.Results:XHS can significantly reduce the frequency of cough and increase the amount of phenol red in the trachea of mice,improve the pathological changes of acute rhinitis in mice,and reduce inflammation and bleeding.XHS can significantly inhibit the release of pro-inflammatory mediators and has obvious antibacterial activity against major respiratory pathogens in vitro,showing good anti-inflammatory and antibacterial effects in vitro.Conclusion:XHS has the pharmacological effects of relieving cough and resolving phlegm,anti-inflammation and antibacterial.It can significantly improve the pathological changes of acute rhinitis in mice,reduce inflammation and bleeding,and has a certain prevention and treatment effect on upper respiratory tract infection.

关 键 词：香藿喷雾剂 上呼吸道感染 止咳化痰 急性鼻炎 抑菌 抗炎 

分 类 号：R965[医药卫生—药理学]