基于宏基因组技术研究亚慢性氯仿暴露对小鼠肠道微生物群的影响  

Macrogenomic-based Study of Effects of Subchronic Chloroform Exposure on the Gut Microbiota of Mice

作　　者：王再山 李强 栾天琪 金志民[1] 刘铸[1] WANG Zaishan;LI Qiang;LUAN Tianqi;JIN Zhimin;LIU Zhu(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,牡丹江157011

出　　处：《生态毒理学报》2025年第1期419-431,共13页Asian Journal of Ecotoxicology

基　　金：黑龙江省自然科学基金项目(LH2021C095);黑龙江省省属高等学校基本科研业务费科研项目(1452TD008,1451TD002)。

摘　　要：肠道微生物群是一个复杂的共生细菌生态系统,与宿主健康状况,代谢表型,营养吸收或产生,以及免疫系统调节有关。氯仿是1种城市常见的有毒的环境污染物,人类可能通过包括空气或自来水在内的各种媒介接触到氯仿。在本研究中,将9只SPF级(无特定病原体)昆明雌性小鼠分为3组(每组3只),3组灌胃分别暴露于0、50和150 mg·kg^(-1)小鼠体质量的剂量中,持续30 d。我们的实验结果揭示了以下几点:(1)亚慢性氯仿暴露显著改变了盲肠微生物群的物种组成和结构。在科水平上,在氯仿暴露组中,乳杆菌科(Lactobacillaceae)、康氏菌科(Kangiellaceae)等细菌的相对丰度显著降低。基于LEfSe线性判别分析表明,臭杆菌科(Odoribacteraceae)可作为氯仿暴露组的典型生物标志物。在属水平上,氯仿暴露使螺杆菌属(Helicobacter)、颤杆菌克属(Oscillibacter)等细菌的相对丰度显著增加,且与氯仿剂量呈正相关。(2)氯仿的暴露显著影响了微生物群功能基因的组成。基于代谢通路水平功能比较分析表明,花生四烯酸代谢和类固醇生物合成的相对丰度在对照组显著高于氯仿实验组,并与氯仿浓度的呈负相关;基于碳水化合物活性酶水平功能比较分析表明,如GH31、GT14、GT113、GH67等碳水化合物活性酶的相对丰度由于氯仿的暴露显著降低;基于抗生素抗性基因分析表明,如ARO:3000412、ARO:3000569、ARO:3001209、ARO:3002894等抗性基因的相对丰度在氯仿暴露高于对照组,且与氯仿剂量呈正相关。综上所述,我们的研究结果表明亚慢性氯仿暴露显著影响了小鼠盲肠微生物群的物种的组成结构,并通过扰乱肠道微生物群的组成结构,从而影响了花生四烯酸代谢、类固醇生物合成等新陈代谢,以及碳水化合物分解消化能力和抗生素抗性基因的组成。The intestinal microbiota represents a sophisticated symbiotic bacterial ecosystem intricately linked to host health status,metabolic phenotypes,nutrient absorption or generation,and immune system modulation.Chloroform,a prevalent urban toxic environmental pollutant,can be encountered by humans through various vectors,including air and tap water.In this investigation,nine SPF Kunming female mice were stratified into three cohorts(3 mice per cohort)and subjected to oral gavage with chloroform doses of 0,50,and 150 mg·kg^(-1)of body weight,respectively,over 30 d.Our empirical observations reveal the following:(1)Subacute chloroform exposure induces significant alterations in the taxonomic composition and structure of the cecal microbiota.Specifically,chloroform exposure results in a notable reduction in the relative abundance of bacterial families such as Lactobacillaceae and Kangiellaceae.Linear discriminant analysis effect size(LEfSe)identifies Odoribacteraceae as a potential signature taxon for the chloroform-exposed group.At the genus level,chloroform exposure correlates positively with the increased relative abundance of taxa such as Helicobacter and Oscillibacter.(2)Chloroform exposure exerts a discernible influence on the repertoire of functional microbial gene pathways.Comparative metabolic pathway analysis reveals a significantly elevated relative abundance of pathways associated with arachidonic acid metabolism and steroid biosynthesis in the control group compared to the chloroform-exposed group,exhibiting a negative correlation with chloroform dosage.Evaluation of carbohydrate-active enzyme profiles demonstrates a marked decrease in the relative abundance of enzyme genes such as GH31,GT14,GT113,and GH67 following chloroform exposure.Examination of antibiotic resistance gene profiles highlights an increased relative abundance of resistance genes,including ARO:3000412,ARO:3000569,ARO:3001209,and ARO:3002894,in the chloroform-exposed group relative to the control group,positively associated with chlorofor

关 键 词：氯仿 环境污染物 肠道微生物群 宏基因组 物种多样性 功能基因多样性 

分 类 号：X171.5[环境科学与工程—环境科学]