沙棘多糖对环磷酰胺所致小鼠免疫功能低下的缓解作用  

Polysaccharide from Hippophae rhamnoides L.Alleviates Immunosuppression Induced by Cyclophosphamide in Mice

作　　者：江峻峰 包晓玮 孙嘉莉 敖羽 宋素琴[2] JIANG Junfeng;BAO Xiaowei;SUN Jiali;AO Yu;SONG Suqin(School of Food Science and Pharmacy,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;Xinjiang Key Laboratory of Special Environmental Microorganisms,Institute of Microbiology Application,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,Xinjiang,China)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052 [2]新疆农业科学院微生物应用研究所新疆特殊环境微生物重点实验室,新疆乌鲁木齐830091

出　　处：《食品研究与开发》2025年第4期26-32,共7页Food Research and Development

基　　金：自治区重点研发计划项目(2022B02005-4)。

摘　　要：为评价沙棘多糖(polysaccharide from Hippophae rhamnoides L.,PHR)对环磷酰胺(cyclophosphamide,CTX)导致免疫抑制小鼠的免疫功能低下的缓解作用,以分离制备的PHR为供试品,将小鼠分为空白组、环磷酰胺模型组、PHR低、中、高(100、200、400 mg/kg)剂量组。利用环磷酰胺建立免疫抑制小鼠模型后,灌胃PHR,利用体质量称量、碳廓清、酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)、甲基噻唑基四唑盐比色法[(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,MTT)]测定各组动物免疫器官指数、吞噬指数、血清免疫球蛋白G(immunoglobulin G,IgG)、免疫球蛋白M(immunoglobulin M,IgM)、肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)、γ干扰素(interferon-gamma,IFN-γ)、白细胞介素6(interleukin-6,IL-6)及脾淋巴细胞增殖能力。结果表明,与空白组比较,模型组免疫器官指数、吞噬指数、血清中IgG、IgM、TNF-α、IFN-γ、IL-6含量、淋巴细胞增殖能力均极显著降低(P<0.01),与模型组比较,PHR各剂量组对以上指标均有所改善,其中PHR中、高剂量组的作用效果显著(P<0.05)。因此PHR可以缓解环磷酰胺导致的免疫抑制小鼠的免疫功能低下。To evaluate the immunoregulatory effect of polysaccharide from Hippophae rhamnoides L.(PHR)on immune dysfunction in cyclophosphamide(CTX)-induced immunosuppressed mice,PHR isolated and pre-pared from H.rhamnoides L.was used as the test sample.Mice were divided into blank group,CTX model group,and low-,medium-,and high-dose PHR groups(100,200,and 400 mg/kg).The immunosuppressed mouse model was established using CTX,followed by PHR administration via gavage.The immune organ in-dex,phagocytosis index,serum levels of immunoglobulin G(IgG),immunoglobulin M(IgM),tumor necrosis factor-alpha(TNF-α),interferon-gamma(IFN-γ),interleukin-6(IL-6),and the proliferation ability of spleen lymphocytes in each group were measured using weighing,carbon clearance,enzyme-linked immunosorbent as-say(ELISA),and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.The results showed that compared with the blank group,the model group exhibited significantly reduced immune organ in-dex,phagocytosis index,serum levels of IgG,IgM,TNF-α,IFN-γ,IL-6,and lymphocyte proliferation capacity(P<0.01).Compared with the model group,each dose group of PHR showed improvement in the above in-dexes,with the medium-and high-dose groups demonstrating significantly greater effects(P<0.05).Therefore,PHR can alleviate immune dysfunction in CTX-induced immunosuppressed mice.

关 键 词：沙棘 多糖 免疫抑制 缓解 免疫功能 

分 类 号：TS201.4[轻工技术与工程—食品科学] R285.5[轻工技术与工程—食品科学与工程]