甘蓝型油菜BnaC3.CCD4转基因白花株系白天花色变化的转录组分析  

Transcriptomic analysis on the flower color changes of white-petaled BnaC3.CCD4-transgenic rapeseed(Brassica napus)lines during the daytime

作　　者：王锦婷 孙雪雨 胡文竞 杨谦 刘超[1] WANG Jin-ting;SUN Xue-yu;HU Wen-jing;YANG Qian;LIU Chao(National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University/Hubei Hongshan Laboratory,Wuhan 430070,China)

机构地区：[1]华中农业大学作物遗传改良全国重点实验室/洪山实验室,湖北武汉430070

出　　处：《中国油料作物学报》2025年第1期45-59,共15页Chinese Journal of Oil Crop Sciences

基　　金：武汉市生物育种关键技术攻关及新品种培育科技重大专项(2022021302024851)。

摘　　要：在前期研究中我们发现,BnaC3.CCD4编码类胡萝卜素裂解双加氧酶,该基因的突变导致甘蓝型油菜花色由白色转变为黄色。本研究发现BnaC3.CCD4转基因白花株系的花色在上午为浅黄色,下午才呈现为纯白色,其类胡萝卜素含量显著降低。为探究其花色变化的相关机制,对其分别进行转录组测序分析,GO和KEGG结果显示,其差异基因显著富集到光信号、昼夜节律、刺激响应以及类胡萝卜素生物合成等相关通路。进一步通过分析差异倍数前50的差异基因,发现光信号变化可能是BnaC3.CCD4转基因株系白天花色变化的关键因素。因此推测光信号因子可能通过与类胡萝卜素生物合成通路上关键基因的光响应元件结合,调控了它们的表达量从而影响了花瓣中类胡萝卜素的含量,进而导致白天油菜花色的变化。Our previous study revealed that BnaC3.CCD4 encodes carotenoid cleavage dioxygenas whose mutations altered flower color from white to yellow in rapeseed.Interestingly,we observed that the white flowers of functional BnaC3.CCD4 transgenic lines showed pale yellow in the morning and still became pure white in the afternoon during the daytime.In order to explore whether environmental signals such as light and temperature have a regulatory effect on carotenoid biosynthesis and metabolism,RNA-seq was performed for transcriptomic analysis on them at two different timepoints in the present research.The analysis results of gene ontology(GO)and kyoto encyclopedia of genes and genomes(KEGG)revealed that a number of differential expression genes were significantly enriched in pathways related to light signal,circadian rhythm,stimulus response and carotenoid biosynthesis et al.Through further analyzing the top 50 differentially expressed genes,it was revealed that the light signal might be the key factor for the flower color change of BnaC3.CCD4 transgenic lines.Therefore,it was inferred that the light signal factors potentially regulate the expressions of the genes in the carotenoid biosynthesis pathway by binding to their photoresponsive elements to control the carotenoid contents in the petals and then cause their color change.

关 键 词：甘蓝型油菜 花色 类胡萝卜素裂解双加氧酶 光信号 昼夜节律 

分 类 号：S565.4[农业科学—作物学]