基于线粒体DNA ND6基因序列分析巨魾群体遗传多态性  

Analysis of Bagarius yarrelli Genetic Polymorphism Based on Mitochondrial DNA ND6 Gene

作　　者：杜民 牛宝珍 施来凤 雷美榕 普文华 彭跃颖 张志仙 周甜 古金华 DU Min;NIU Baozhen;SHI Laifeng;LEI Meirong;PU Wenhua;PENG Yueying;ZHANG Zhixian;ZHOU Tian;GU Jinhua(College of Life and Environmental Sciences,Hunan University of Arts and Science/Hunan Provincial Key Laboratory for Health Aquaculture and Product Processing in Dongting Lake Area/Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases/Changde Key Innovation Team for Health Aquaculture and Product Processing/Changde Key Innovation Team for Wetland Biology and Environmental Ecology,Changde 415000,Hunan,China;Hunan Applied Technology University,College of Agriculture and Forestry Science and Technology/Key Laboratory of Featured Aquaculture,Changde 415000,Hunan,China;Honghe University/Key Laboratory of Crops with High Quality and Efficient Cultivation and Security Control,Yunnan Higher Education Institutions,Mengzi 661199,Yunnan,China)

机构地区：[1]湖南文理学院生命与环境科学学院/环洞庭湖水产健康养殖及加工湖南省重点实验室/水生动物重要疫病分子免疫技术湖南省重点实验室/常德市水产养殖与加工重点创新团队/常德市湿地生物与环境生态重点创新团队,湖南常德415000 [2]湖南应用技术学院农林科技学院特色水产重点实验室,湖南常德415000 [3]红河学院/云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661199

出　　处：《四川农业大学学报》2025年第1期33-38,共6页Journal of Sichuan Agricultural University

基　　金：湖南文理学院博士启动项目(19BSQD39);国家自然科学基金(31360638)。

摘　　要：【目的】旨在研究巨魾种质资源、物种间的亲缘关系及遗传多样性。【方法】以云南省3条水系(元江、怒江、澜沧江)5个种群共60尾巨魾为研究对象,选取线粒体ND6基因作为分子标记,对巨魾遗传多样性进行研究。【结果】巨魾线粒体ND6基因序列全长525 bp,60条序列获得41种单倍型;41种单倍型序列中A、T、C、G平均含量分别为40.0%、13.7%、34.8%、11.5%,A+T含量为53.7%,C+G的含量为46.3%;共检测到143个变异位点,375个保守位点,114个简约信息位点;核苷酸多样性和单倍型多样性分别为0.08769±0.00235和0.960±0.017;41种单倍型间平均遗传距离为0.101。【结论】巨魾41种单倍型与其他7种鱼类ND6基因序列用NJ法构建系统发育树,发现巨魾单独聚为一支。【Objective】This study aims to investigate the germplasm resources,genetic relationship,and genetic diversity of Bagarius yarrelli.【Method】The genetic diversity of B.yarrelli was studied by using mitochondrial ND6 gene of 60 individuals as a molecular marker in five populations of Bagariusyarrelli from the three rivers(Yuanjiang,Nujiang,and Lancangjiang)in Yunnan Province.【Result】The results showed that the total length of mitochondrial ND6 was 525 bp,and 41 haplotypes were defined from 60 sequences.The average contents of A,T,C and G in all samples was 40.0%,13.7%,34.8%,and 11.5%,respectively.The A+T content was 53.7%and the percentage of C+G content was 46.3%.A total of 143 mutation sites,375 conserved sites,and 114 parsimony informative sites were detected.The nucleotide diversity and haplotype diversity were 0.08769±0.00235 and 0.960±0.017,respectively;and the average genetic distance between 41 haplotypes in 60 individuals was 0.101.【Conclusion】A phylogenetic tree was constructed using the Neighbor-Joining(NJ)method for the ND6 gene sequence of 41 haplotypes of B.yarrelli and 7 other species of fish.It was found that B.yarrelli clustered into a single branch.

关 键 词：巨魾 线粒体ND6 多态性 系统进化 

分 类 号：S917.4[农业科学—水产科学]