湿地松抗褐变和易褐变无性系胚性愈伤团的生理生化特征  

Physiological and Biochemical Characteristics of Embryogenic Callus Derived from Anti-browning and Easy-browning Lines of Pinus elliottii

作　　者：刘阳 郭文冰[1] 薛蕾 王哲 曾明 欧阳曦 伍彩云 车晓亮 LIU Yang;GUO Wenbing;XUE Lei;WANG Zhe;ZENG Ming;OUYANG Xi;WU Caiyun;CHE Xiaoliang(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization/Guangdong Academy of Forestry,Guangzhou,Guangdong 510520,China)

机构地区：[1]广东省森林培育与保护利用重点实验室/广东省林业科学研究院,广东广州510520

出　　处：《林业与环境科学》2025年第1期9-21,共13页Forestry and Environmental Science

基　　金：国家自然科学基金项目(32101513)。

摘　　要：为明确湿地松体细胞胚胎发生过程中胚性愈伤团褐变的原因,解析褐变的发生机制,研究系统探究了湿地松Pinus elliottii胚性愈伤团的生理生化特征,特别是抗褐变和易褐变无性系之间的差异。通过对胚性愈伤团中总酚含量、多酚氧化酶活性、查尔酮异构酶(CHI)活性、内源激素含量以及黄酮/酚类化合物等的综合分析,揭示了这些因素与褐变之间的潜在关联。结果表明,尽管总酚含量在不同无性系间无显著差异,但CHI活性在易褐变无性系中更高,可能与某些特定黄酮类物质的积累有关。内源激素分析显示IAA、iP+iPR、GA3和JA-me的水平变化可能与褐变密切相关。最后,研究分析了58种黄酮/酚类化合物的积累模式,并进一步挖掘获得了儿茶素、香橙素、原花青素B3、原花青素B1、金丝桃苷、乔松素等褐变特异积累物质。研究发现特定的酶活性、内源激素及黄酮/酚类化合物的变化与胚性愈伤团褐变密切相关,为开发新型褐变调控策略提供了理论基础。To clarify the causes of browning during the somatic embryogenesis of Pinus elliottii and to explain the mechanisms behind this process,this study systematically explores the physiological and biochemical characteristics of embryogenic callus of P.elliottii,particularly the differences between anti-browning and easybrowning lines.Through comprehensive analysis of total phenol content,polyphenol oxidase(PPO)activity,chalcone isomerase(CHI)activity,endogenous hormone levels,and flavonoid/phenolic compounds,the potential correlations between these factors and browning were revealed.The results indicate that although there are no significant differences in total phenol content among different lines,CHI activity is higher in easy-browning lines,which may be related to the accumulation of certain specific flavonoids.Endogenous hormone analysis shows that the level changes of IAA,iP+iPR,GA3,and JA-me may be closely related to browning.Finally,the study analyzes the accumulation patterns of various flavonoid/phenolic compounds and further identifies browning-specific accumulated compounds such as catechin,aromadendrin,procyanidin B3,procyanidin B1,quercetin 3-galactoside,(S)-pinocembrin.This study found that variations in specific enzyme activities,endogenous hormones,and flavonoid/phenolic compounds are closely associated with the browning of embryogenic calluses,thereby providing a theoretical foundation for the development of novel strategies for browning control.

关 键 词：湿地松 体细胞胚胎发生 胚性愈伤团 褐变 

分 类 号：S791.24[农业科学—林木遗传育种]