树干注射甲维盐制剂对松材线虫病林间防效的综合评价  

Comprehensive Evaluation About Control Effect of Trunk Injections of Emaectin Benzoate on Pine Wilt Disease in the Field

作　　者：宋高飞 张标强 童浩 蓝文丰 张治昊 汤陈生[5] 蔡守平[5] SONG Gaofei;ZHANG Biaoqiang;TONG Hao;LAN Wenfeng;ZHANG Zhihao;TANG Chensheng;CAI Shouping(Syngenta(Shanghai)Crop Protection Technology Co.,Ltd,Shanghai 200126,China;Guanzhuang State-owned Forestry Farm,Sanming,Fujian 365050,China;Shuinan State-owned Forestry Farm,Sanming,Fujian 365050,China;Wuyi Stateowned Forestry Farm,Zhangping,Longyan,Fujian 364400,China;Fujian Academy of Forestry/The Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China,Fuzhou,Fujian 350012,China)

机构地区：[1]先正达(上海)作物保护科技有限公司,上海200126 [2]福建省沙县官庄国有林场,福建三明365050 [3]福建省沙县水南国有林场,福建三明365050 [4]福建省漳平五一国有林场,福建龙岩364400 [5]福建省林业科学研究院/国家林业和草原局南方山地用材林培育重点实验室,福建福州350012

出　　处：《林业与环境科学》2025年第1期86-93,共8页Forestry and Environmental Science

基　　金：福建省林业科技项目(ZMGG-0705)。

摘　　要：为了明确高压注射2%甲维盐乳油对林间松材线虫病的防治效果,研究分析了注射甲维盐后林间马尾松Pinus massoniana体内残留量的时间动态变化及甲维盐对树体内松材线虫Bursaphelenchus xylophilus种群的抑制作用,并研究了注射甲维盐对寄主马尾松活性氧(Reactive oxygen species,ROS)水平和功能微生物的影响,从不同层面综合评价了甲维盐对林间松材线虫病的直接和间接防治效果。结果表明,甲维盐注干18个月后,试验区马尾松植株保存率超过98%。注射甲维盐1 a后,马尾松树体内树干和枝条均可检测到残留,在树体上部、中部及冠层枝条呈下降趋势,树体下部在试验期间呈上升趋势且马尾松体内残留量均高于LC_(95)(0.05μg·g^(-1));注射甲维盐后,室内外马尾松植株体内松材线虫数量较对照显著下降(P<0.05),室内马尾松幼苗体内松材线虫数量下降61.02%,林间松材线虫高携带单株(≥5000)和低携带单株(<5000)体内松材线虫携带量分别减少93.01%和71.09%;注射甲维盐能显著提升马尾松植株体内ROS水平(P<0.05),室内接种松材线虫的马尾松幼苗、林间松材线虫高携带量和低携带量的马尾松个体的ROS水平分别为对照的7.96、1.90和6.59倍;马尾松中抗性相关微生物Penicillium的丰度均显著提高(P<0.05),松材线虫携带量高和低的马尾松体内较对照分别提升19.12%和3.15%。研究结果表明了树干注射甲维盐2%乳油可显著林间降低马尾松植株体内的松材线虫数量,并提升寄主马尾松的活性氧水平及Penicillium属丰度。To clarify the effect of high-pressure injection of 2%Emamectin benzoate(EB)on the control of pine wilt disease(PWD)in the forest,this study analyzed the temporal dynamics of residues in Pinus massoniana and the inhibitory effect of EB on Bursaphelenchus xylophilus populations in the trees,and also studied the impact of EB on the levels of reactive oxygen species(ROS)and functional microorganisms in the host P.massoniana.From different perspectives,the study comprehensively evaluated the direct and indirect control effects of EB on PWD in forests.The results showed that the preservation rate of P.massoniana plants in the experimental area was over 98%after 18 months of EB injection.One year after the injection of EB,residues could be detected in the trunks and branches of P.massoniana trees,with a decreasing trend in the upper,middle parts and canopy branches,and an increasing trend in the lower part of the tree during the test period.The residues levels in P.massoniana trees were higher than LC_(95)(0.05μg·g^(-1)).After the injection of EB,the number of B.xylophilus in P.massoniana plants indoors and outdoors decreased significantly compared to the control(P<0.05).The number of B.xylophilus in indoor P.massoniana seedlings decreased by 61.02%,and the number of B.xylophilus in P.massoniana with high(≥5000 nematodes)and low(<5000 nematodes)burdens of B.xylophilus in the forest decreased by 93.01%and 71.09%,respectively.The injection of EB significantly increased the ROS level in P.massoniana plants(P<0.05),and the ROS levels in indoor P.massoniana seedlings inoculated with B.xylophilus,and in individual P.massoniana trees with high and low burdens of B.xylophilus in forest stands,were 7.96,1.90,and 6.59 times that of the control,respectively.The abundance of Penicillium,a resistance-associated microorganism,was significantly higher(P<0.05)in both P.massoniana seedlings and trees,and increased by 19.12%and 3.15%,respectively,in trees with with high and low burdens of B.xylophilus compared to the control.The res

关 键 词：松材线虫病 马尾松 2%甲维盐乳油 残留量 ROS 防治效果 

分 类 号：S763.7[农业科学—森林保护学]