QuEChERS结合GC-MS/MS法测定蕹菜中41种农药残留  

Determination of 41 Pesticide Residues in Water Spinach by QuEChERS Combined with GC-MS/MS

作　　者：李南 韦盈 王丹 吴含秀 陈思明 智丽 LI Nan;WEI Ying;WANG Dan;WU Hanxiu;CHEN Siming;ZHI Li(Wuzhishan Branch of Hainan Academy of Inspection and Testing,Wuzhishan 572200,China;Institute of Food Testing,Hainan Academy of Inspection and Testing,Key Laboratory of Tropical Fruits and Vegetables Quality and Safety,State Administration for Market Regulation,Haikou 570314,China)

机构地区：[1]海南省检验检测研究院五指山分所,海南五指山572200 [2]海南省检验检测研究院食品检验检测中心,国家市场监督管理总局重点实验室(热带果蔬质量与安全),海南海口570314

出　　处：《现代食品》2025年第1期183-188,195,共7页Modern Food

基　　金：国家市场监督管理总局重点实验室(热带果蔬质量与安全)项目(KF-2024012)。

摘　　要：建立测定蕹菜中41种农药残留量的QuEChERS-气相色谱-串联质谱(Gas Chromatography-Tandem Mass Spectrometry,GC-MS/MS)检测方法。蕹菜样品经乙腈提取后,采用QuEChERS方法前处理,以N-丙基乙二胺(N-Propyl Ethylenediamine,PSA)、石墨化炭黑(Graphitized Carbon Black,GCB)吸附提取液中的干扰组分,提取液经GC-MS/MS多反应监测模式分析测定,匹配基质标准曲线内标法定量。结果表明,采用本研究建立的方法测定41种农药,线性范围为10~500 ng·mL^(-1),相关系数(R^(2))在0.9938~0.9998,线性关系良好,定量限均为0.01 mg·kg^(-1)。在0.010、0.025、0.250 mg·kg^(-1)3个加标水平下的平均加标回收率为62.1%~117.2%,相对标准偏差在0.2%~14.3%(n=6)。所建方法的准确度、精密度均能满足蕹菜中41种农药残留基本定量要求,操作简单、效率高,可用于蕹菜中实际农药残留的检测。Establish a QuEChERS gas chromatography-tandem mass spectrometry(GC-MS/MS)detection method for the determination of 41 pesticide residues in water spinach.After extracting the water spinach samples with acetonitrile,the QuEChERS method was used for pretreatment.N-propyl ethylenediamine(PSA)and graphitized carbon black(GCB)were used to adsorb interfering components in the extraction solution.The extraction solution was analyzed and determined by GC-MS/MS multi reaction monitoring mode,and quantified by matching the matrix standard curve internal standard method.The results showed that the method established in this study was used to determine 41 pesticides,with a linear range of 10~500 ng·mL^(-1) and a correlation coefficient(R^(2))were 0.9938~0.9998.The linear relationship was good,and the limit of quantification was 0.01 mg·kg^(-1) for all.The average spiked recovery rates at three dosages of 0.010 mg·kg^(-1),0.025 mg·kg^(-1),and 0.250 mg·kg^(-1) were between 62.1%and 117.2%,with relative standard deviations ranging from 0.2%to 14.3%(n=6).The accuracy and precision of the established method can meet the basic quantitative requirements of 41 pesticide residues in water spinach,with simple operation and high efficiency,and can be used for the detection of actual pesticide residues in water spinach.

关 键 词：QUECHERS 气相色谱-三重四极杆串联质谱 基质效应 

分 类 号：TS207.3[轻工技术与工程—食品科学]