鼻咽癌组织驱动蛋白超家族18B、20A与上皮间质转化标志物及临床病理特征和预后的关系  

The relationship between kinesin superfamily 18B,20A and epithelial-mesenchymal transition markers,clinicopathological features and prognosis in nasopharyngeal carcinoma

作　　者：白芸芸 贾乐[2] 陈福权 BAI Yunyun;JIA Le;CHEN Fuquan(Department of Otolaryngology,Yulin Traditional Chinese Medicine Hospital,Yulin,Shaanxi,719000,China;Department of Pathology,Baoji Traditional Chinese Medicine Hospital,Baoji,Shaanxi,721000,China;Department of Otolaryngology,Air Force Medical University Xijing Hospital,Xi'an,Shaanxi,710000,China)

机构地区：[1]榆林市中医医院耳鼻咽喉科,陕西榆林719000 [2]宝鸡市中医医院病理科,陕西宝鸡721000 [3]空军军医大学西京医院耳鼻咽喉科,陕西西安710000

出　　处：《中国耳鼻咽喉头颈外科》2025年第1期1-7,共7页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：陕西省卫生健康科研项目(2021D015)。

摘　　要：目的探讨鼻咽癌组织驱动蛋白超家族(KIF)18B、20A与上皮间质转化(EMT)标志物以及临床病理特征和预后的关系。方法选取2017年7月~2019年5月榆林市中医医院耳鼻咽喉科病理诊断为鼻咽癌的116例鼻咽活检肿瘤组织,以116例鼻咽慢性黏膜炎症患者的鼻黏膜活检组织作为对照,比较鼻咽癌组织和鼻咽慢性黏膜炎症组织中KIF18B、KIF20A以及EMT标志物上皮钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)、扭曲蛋白(Twist)表达,分析鼻咽癌组织中KIF18B、KIF20A表达与EMT标志物表达及临床病理特征的关系。随访5年,采用Kaplan-Meier法及多因素Cox回归分析KIF18B、KIF20A表达与鼻咽癌患者预后的关系。结果鼻咽癌组织的KIF18B、KIF20A、Vimentin、Twist阳性表达率分别为55.17%、42.24%、20.69%、26.72%,高于鼻咽慢性黏膜炎症组织的13.79%、9.48%、4.31%、5.17%,鼻咽癌组织的E-cadher in阳性表达率为68.10%,低于鼻咽慢性黏膜炎症组织的91.38%(P<0.05)。KIF18B、KIF20A与E-cadherin表达呈负相关(r_(s)=-0.439、-0.432,P均<0.05),KIF18B、KIF20A与Vimentin(r_(s)=0.418、0.456,P均<0.05)、Twist呈正相关(r_(s)=0.427、0.427,P均<0.05)。KIF18B、KIF20A的阳性表达率在低分化、有淋巴结转移、TNM分期Ⅲ~Ⅵa期、临床疗效疾病稳定(SD)/疾病进展(PD)高于中高分化、无淋巴结转移、Ⅰ~Ⅱ期和完全缓解(CR)/部分缓解(PR),差异有统计学意义(P<0.05)。随访5年共4例患者失访,随访成功率为96.55%(112/116),死亡31例,5年总体生存率为72.32%(81/112)。KIF18B、KIF20A阳性表达鼻咽癌患者的5年累积生存率低于KIF18B、KIF20A阴性患者(P<0.05)。TNM分期Ⅲ~Ⅵa期(HR=1.154,95%CI:1.018~1.308)、临床疗效SD/PD(HR=1.287,95%CI:1.132~1.654)、KIF18B(HR=1.823,95%CI:1.332~2.493)和KIF20A阳性表达(HR=2.110,95%CI:1.460~3.051)均是影响鼻咽癌患者预后的独立危险因素(P<0.05)。结论鼻咽癌组织中KIF18B、KIF20A异常高表达,两者阳性表达与EMT、肿瘤恶性OBJECTIVE To investigate the relationship between kinesin superfamily(KIF)18B,20A and epithelialmesenchymal transition(EMT)markers,clinicopathological features and prognosis in nasopharyngeal carcinoma.METHODS 116 cases of nasopharyngeal biopsy tumor tissues diagnosed as nasopharyngeal carcinoma in Department of Otolaryngology,Yulin Traditional Chinese Medicine Hospital from July 2017 to May 2019 were selected,nasal mucosa biopsy tissues of 116 chronic nasopharyngitis patients were used as control,the expression of KIF18B,KIF20A,and EMT markers epithelial calreticulin(E-cadherin),vimentin,twist in nasopharyngeal carcinoma tissues and nasopharyngeal chronic mucositis tissues were compared.The relationship between KIF18B,KIF20A expression and EMT marker expression and clinicopathological features in nasopharyngeal carcinoma tissues were analyzed.5 years after follow-up,the relationship between KIF18B,KIF20A expression and the prognosis of nasopharyngeal carcinoma patients were analyzed by the Kaplan-Meier method and multivariate Cox regression analysis.RESULTS The positive expression rates of KIF18B,KIF20A,Vimentin and Twist in nasopharyngeal carcinoma tissues were 55.17%,42.24%,20.69%,and 26.72%respectively,which were higher than 13.79%,9.48%,4.31%,and 5.17%in nasopharyngeal chronic mucosal inflammation tissues;The positive expression rate of E-cadherin in nasopharyngeal carcinoma tissues was 68.10%,which was lower than 91.38%in nasopharyngeal chronic mucosal inflammation tissues(P<0.05).KIF18B and KIF20A were negatively correlated with E-cadherin expression(r_(s)=-0.439,-0.432,P<0.05),KIF18B,KIF20A were positively correlated with Vimentin(r_(s)=0.418,0.456,P<0.05)and Twist(r_(s)=0.427,0.427,P<0.05).The KIF18B and KIF20A positive expression rates in patients with low differentiation,lymph node metastasis,TNM stage III-VIa,clinical efficacy stable disease(SD)/progressive disease(PD)were higher than those in patients with high differentiation,no lymph node metastasis,stage I-II,and complete response(CR)/partial resp

关 键 词：鼻咽肿瘤 预后 驱动蛋白超家族18B 驱动蛋白超家族20A 上皮间质转化 临床病理特征 

分 类 号：R739.63[医药卫生—肿瘤]