不同赏食两用百合营养价值分析及其种苗快繁体系的优化  

Analysis of nutritional value of lily and optimization of rapid propagation system

作　　者：庞晓静 马晨晨 王贞红 王磊[1] 任叶飞 程华 王云生[1] PANG Xiaojing;MA Chenchen;WANG Zhenhong;WANG Lei;REN Yefei;CHENG Hua;WANG Yunsheng(School of Life Sciences,Anhui Agricultural University,Hefei 230036;Resources&Environment College,Tibet Agricultural and Animal Husbandry University,Linzhi 860000;Anhui Mengsikang Chinese Herbal Medicine Co.,Ltd.,Tongling 244000)

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]西藏农牧学院资源与环境学院,林芝860000 [3]安徽梦思康中药材有限公司,铜陵244000

出　　处：《安徽农业大学学报》2024年第6期929-935,共7页Journal of Anhui Agricultural University

基　　金：产学研合作项目(KJ2021454)资助。

摘　　要：以5种不同颜色的赏食两用百合(白色、黄色、橙黄色、紫红色和黄红复色)为材料,分析了供试材料田间表型及鳞茎中营养成分的差异,优化其组织培养快繁体系。田间表型分析表明,除花色不同外,供试百合品种株高存在显著差异。其中,白色百合植株最高,黄红复色百合植株最矮。从功能性成分上看,橙黄色百合营养价值极具特色,其多糖、总酚酸和总皂苷含量在测定品种中最高,含量分别达50.47、11.56和2.83 mg·g^(-1);黄红复色百合具有较高的抗氧化潜力,其总黄酮含量最高,为3.10 mg·g^(-1)。通过对培养条件和培养基优化,确定了百合鳞茎外植体最佳消毒方式为5%次氯酸钠消毒10 min;百合不定芽诱导的最适培养基激素浓度为1.0 mg·L^(-1)6-BA和0.2 mg·L^(-1)NAA;不定芽增殖的最适培养基激素浓度为2.0 mg·L^(-1)6-BA和1.0mg·L^(-1)NAA;百合生根的最适培养基为浓度0.3mg·L^(-1)IBA和0.5 mg·L^(-1)NAA的MS培养基。研究结果为赏食两用百合健康价值挖掘及种苗扩繁、推广奠定了理论基础。In this study,the differences in field phenotype and nutritional composition of the bulbs of five differ-ent colors of the dual-use lilies(white,yellow,orange-yellow,fuchsia and yellow-red)were analyzed,and the tissue culture and rapid propagation system were optimized.The results showed that there were significant differences in plant height among the tested lily varieties except for the flower color.Among them,the white lily plant height was the highest,and yellow-red compound color lily plant height was the lowest.In terms of functional composition,the nutri-tional value of orange-yellow lilies was very distinctive,with the highest contents of polysaccharides,total phenolic acids,and total saponins among the assayed varieties,which amounted to 50.47,11.56 and 2.83 mg·g^(-1),respectively;the yellow-red composite-color lilies had a relative high antioxidant potential,with the highest total flavonoids content,which amounted to 3.10 mg·g^(-1).Through the optimization of culture conditions and media,the optimal disinfection method of Lily bulbs explants was observed as a 10-min treatment with 5%sodium hypochlorite.For the induction of lily adventitious buds,the optimum hormone concentrations were found to be 1.0 mg·L^(-1)6-BA and 0.2 mg·L^(-1) NAA,while for bud proliferation,it was 2.0 mg·L^(-1)6-BA and 1.0 mg·L^(-1) NAA.Lily rooting was most successful in a me-dium with concentrations of 0.3 mg·L^(-1) IBA and 0.5 mg·L^(-1) NAA in the MS culture medium.The above research re-sults lay a theoretical foundation for the mining of the health value of edible lily and the promotion of seedlings.

关 键 词：百合 赏食两用 营养成分 种苗快繁 

分 类 号：S644.1[农业科学—蔬菜学]