牵张力对大鼠髁突纤维软骨干细胞成软骨分化的影响  

Effects of stretch on chondrogenic differentiation of rat condylar fibrocartilage stem cells

作　　者：龚吴仪 米晓晖[1] 李永明 GONG Wuyi;MI Xiaohui;LI Yongming(Shanghai Engineering Research Center of Tooth Restoration and Regeneration&Tongji Research Institute of Stomatology&Department of Orthodontics,Shanghai Tongji Stomatological Hospital and Dental School,Tongji University,Shanghai 200072,China)

机构地区：[1]上海市同济口腔医院正畸科,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学口腔医学研究所,上海200072

出　　处：《口腔颌面外科杂志》2025年第1期14-20,共7页Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(81970921)。

摘　　要：目的:观察牵张力对大鼠髁突纤维软骨干细胞(fibrocartilage stem cells,FCSCs)成软骨分化的影响。方法:提取大鼠髁突软骨表层中的FCSCs,通过三系分化诱导验证大鼠髁突FCSCs的多向分化能力,并通过流式细胞术检测其表面标志物的表达情况。然后对大鼠髁突FCSCs施加10%拉伸幅度的牵张力培养48 h后,通过实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)与蛋白质印迹法检测牵张力对成软骨相关基因表达的影响。此外,建立大鼠下颌前导(mandibular advancement,MA)模型以探究体内牵张力对髁突软骨表层的影响。结果:大鼠髁突FCSCs具有向脂肪、骨、软骨多向分化的能力,其表达间充质标志物CD90与CD29,而不表达髓系标志物CD45与CD11。与对照(Control)组相比,牵张加力(Stretch)组的大鼠髁突FCSCs中SRY相关高迁移率族盒蛋白9(SRY-related high mobility group-box 9,Sox9)、蛋白聚糖4(proteoglycan 4,Prg4)与Ⅰ型胶原(typeⅠcollagen,Col1)的基因表达水平显著上升,而基质金属蛋白酶13(matrix metalloproteinase 13,MMP13)的基因表达水平显著降低。与Control组相比,牵张力可以在体内显著增加大鼠髁突软骨表层的厚度。结论:牵张力可以促进大鼠髁突FCSCs的成软骨分化与髁突软骨表层基质合成。Objective:To investigate the effect of mechanical stretch on the chondrogenic differentiation of rat condylar fibrocartilage stem cells(FCSCs).Methods:Triple-lineage induction was used to verify the multi-lineage differentiation ability of rat condylar FCSCs,and flow cytometry was conducted to examine the surface markers of rat condylar FCSCs.Afterward,the rat condylar FCSCs were stretched with a strength of 10%for 48 h,and the expression of related chondrogenic genes was detected by real-time quantitative polymerase chain reaction(RT-qPCR)and Western blotting.We also established rat mandibular advancement(MA)model to find out the in vivo effect of mechanical stretch on the superficial layer of the condylar cartilage.Results:The rat condylar FCSCs could differentiate towards adipogenesis,osteogenesis,and chondrogenesis,and they expressed mesenchymal markers CD90 and CD29,while negative for hemopoietic markers CD45 and CD11.Compared with the Control group,the Stretch group expressed a higher level of SRY-related high mobility group-box 9(Sox9),proteoglycan 4(Prg4),and typeⅠcollagen(Col1)genes,while a lower level of matrix metalloproteinase 13(MMP13)gene.In vivo,mechanical stretch could increase the superficial layer thickness in the rat condylar cartilage compared with the Control group.Conclusion:Mechanical stretch can promote the chondrogenic differentiation of rat condylar FCSCs and the cartilage matrix formation in the superficial layer of the condylar cartilage.

关 键 词：牵张力 髁突 纤维软骨干细胞 成软骨分化 

分 类 号：R782[医药卫生—口腔医学]