胃癌中THBS2 mRNA表达水平与预后及免疫细胞浸润的相关性研究  

Correlation between THBS2 mRNA expression levels and prognosis and immune cell infiltration in gastric cancer

作　　者：王帝 宋展[1] 孙万日[1] Wang Di;Song Zhan;Sun Wanri(Department of General Surgery,Nanyang Central Hospital,Nanyang,Henan 473000,China)

机构地区：[1]南阳市中心医院普外科,河南南阳473000

出　　处：《齐齐哈尔医学院学报》2025年第3期234-238,共5页Journal of Qiqihar Medical University

摘　　要：目的 通过生物信息学数据探讨胃癌中血小板反应蛋白2(THBS2)mRNA表达水平及其临床意义。方法 TCGA数据库下载胃癌的转录组学数据和临床—病理资料数据。胃癌中THBS2 mRNA表达水平采用TPM格式进行转换。胃癌患者不良预后的危险因素采用COX回归分析,胃癌组织中THBS2 mRNA表达水平与总体生存率的关系采用K-M曲线分析,胃癌组织中THBS2 mRNA表达水平与免疫细胞浸润程度的相关性采用Pearson法。免疫组化法分析45例胃癌组织及癌旁组织中THBS2表达水平的差异。结果 与正常胃黏膜组织相比,THBS2 mRNA表达水平在胃癌组织中显著升高[30.85(10.55,71.95)vs.3.13(1.43,9.24),P<0.001]。年龄(>65周岁)、M分期(M1期)、病理分期(Ⅲ~Ⅳ)、THBS2 mRNA表达(高表达)均为影响胃癌患者不良预后的独立危险因素(P<0.05)。相对于胃癌组织中THBS2 mRNA低表达,胃癌组织中THBS2 mRNA高表达生存率显著降低(HR=1.55,P<0.001)。胃癌组织中THBS2 mRNA表达与CD4+T细胞(r=0.211,P<0.001)、CD8+T细胞(r=0.144,P<0.001)、巨噬细胞(r=0.526,P<0.001)、中性粒细胞(r=0.264,P<0.001)和树突状细胞(r=0.393,P<0.001)浸润水平具有显著正相关性(P<0.05),与B细胞浸润(r=-0.169,P<0.001)水平具有显著负相关性。45例胃癌组织中THBS2高度表达30例,THBS2低度表达15例,胃癌癌旁组织中THBS2高度表达4例,THBS2低度表达41例。与癌旁组织相比,THBS2高度表达率在胃癌组织中显著升高(χ2=31.954,P<0.001)。结论 THBS2在胃癌组织中显著高表达,THBS2是胃癌患者发生不良预后的一项生物学标志物。Objective To explore thrombospondin 2(THBS2)mRNA expression levels and its clinical significance based on bioinformatics data.Methods The transcriptomic and clinicopathological data of gastric cancer were downloaded from the TCGA database.THBS2 mRNA expression levels in gastric cancer were transformed using the TPM format.COX regression analysis was used to identify risk factors for poor prognosis in gastric cancer patients.The relationship between THBS2 mRNA expression levels and overall survival rate was analyzed using the Kaplan-Meier curve.The correlation between THBS2 mRNA expression levels and immune cell infiltration was assessed using the Pearson method.Immunohistochemistry was employed to compare THBS2 expression levels between gastric cancer tissues and adjacent tissues in 45 cases.Results Compared to normal gastric mucosa,THBS2 mRNA expression levels were significantly higher in gastric cancer tissues[30.85(10.55,71.95)vs.3.13(1.43,9.24),P<0.001].Age(>65 years),M stage(M1),pathological stage(Ⅲ~Ⅳ),and high THBS2 mRNA expression were all independent risk factors for poor prognosis in gastric cancer patients(P<0.05).Patients with high THBS2 mRNA expression had significantly lower survival rates(HR=1.55,P<0.001).There was a significant positive correlation between THBS2 mRNA expression and the infiltration levels of CD4+T cells(r=0.211,P<0.001),CD8+T cells(r=0.144,P<0.001),macrophages(r=0.526,P<0.001),neutrophils(r=0.264,P<0.001),and dendritic cells(r=0.393,P<0.001).A significant negative correlation was observed with B cell infiltration(r=-0.169,P<0.001).Among the 45 gastric cancer tissues,30 showed high THBS2 expression and 15 showed low expression;in paracancerous tissues,4 showed high expression and 41 showed low expression.The rate of high THBS2 expression was significantly higher in gastric cancer tissues than in paracancerous tissues(χ2=31.954,P<0.001).Conclusions THBS2 is highly expressed in gastric cancer tissues and serves as a biomarker for poor prognosis in gastric cancer patients.

关 键 词：胃癌 血小板反应蛋白2 预后 免疫细胞 浸润 

分 类 号：R73[医药卫生—肿瘤]