三阴性乳腺癌组织中LETM1、HIF-1α的表达及其对临床预后的影响  

Expression of LETM1 and HIF-1 α in triple negative breast cancer tissue and its influence on clinical prognosis

作　　者：李庆辉 牛冰[1] 李海平[1] 冯燕枝 叶贝贝 Li Qinghui;Niu Bing;Li Haiping;Feng Yanzhi;Ye Beibei(Department of Breast Surgery,Zhengzhou People's Hospital,Zhengzhou,Henan 450000,China)

机构地区：[1]郑州人民医院乳腺外科,河南郑州450000

出　　处：《齐齐哈尔医学院学报》2025年第4期327-331,共5页Journal of Qiqihar Medical University

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20220784);河南省医学科技攻关计划联合共建项目(LHGJ20220783)。

摘　　要：目的 探讨三阴性乳腺癌(TNBC)组织中亮氨酸拉链EF-hand结构域跨膜蛋白1(LETM1)、缺氧诱导因子-1α(HIF-1α)的表达及其对临床预后的影响。方法 回顾性分析2018年5月—2021年5月本院收治的177例行改良根治术治疗的TNBC患者病历资料和随访资料,根据术后3年复发情况将其分为复发组(n=53)和未复发组(n=124)。检测TNBC患者手术切除标本的癌组织和癌旁组织中LETM1、HIF-1α表达水平并比较差异。比较不同预后TNBC患者基线资料及癌组织中LETM1、HIF-1α表达;采用Logistic回归分析TNBC患者临床预后的影响因素;绘制受试者工作特征(ROC)曲线,检验癌组织中LETM1、HIF-1α对TNBC患者临床预后的预测价值;绘制决策曲线评估癌组织中LETM1、HIF-1α预测TNBC患者临床预后的临床获益情况。结果 癌组织中LETM1、HIF-1α表达水平高于癌旁正常组织(P<0.05)。复发组和未复发组肿瘤最大径、淋巴结转移、Ki-67、LETM1、HIF-1α比较,差异有统计学意义(P<0.05)。经Logistic回归分析,肿瘤最大径>5 cm、Ki-67阳性、LETM1、HIF-1α是TNBC患者复发的危险因素(OR>1,P<0.05)。绘制ROC曲线显示,癌组织中LETM1、HIF-1α单独及联合对TNBC患者复发均有一定预测价值,且联合预测价值最高(P<0.05)。绘制决策曲线显示,在阈值0.12~0.95范围内,癌组织中LETM1、HIF-1α联合预测TNBC患者复发的净收益率优于单一指标,最大净收益率为0.299;且在高风险阈值0~1范围内,联合预测的净收益率>0,有临床意义。结论 LETM1、HIF-1α在TNBC组织中呈高表达,二者均为TNBC患者复发的影响因素,且可作为患者复发的预测指标,联合两项指标可提高预测效能。Objective To investigate the expression of leucine zipper EF hand domain transmembrane protein 1(LETM1)and hypoxia inducible factor-1α(HIF-1α)in triple negative breast cancer(TNBC)and their effects on clinical prognosis.Methods A retrospective analysis was conducted on the medical records and follow-up data of 177 TNBC patients who underwent modified radical surgery in the hospital from May 2018 to May 2021.They were divided into a recurrence group(n=53)and a non recurrence group(n=124)based on their 3-year postoperative recurrence status.The expression levels of LETM1 and HIF-1αin cancer tissues and adjacent tissues of surgical resection specimens of TNBC patients were detected and compared.The baseline data of TNBC patients with different prognosis and the expression of LETM1 and HIF-1αin cancer tissues were compared.Logistic regression was used to analyze the influencing factors of clinical prognosis in TNBC patients.The receiver operating characteristic(ROC)curve was drawn to test the predictive value of LETM1 and HIF-1αin cancer tissues for the clinical prognosis of TNBC patients.The decision curve was drawn to evaluate the clinical benefits of LETM1 and HIF-1αin cancer tissues in predicting the clinical prognosis of TNBC patients.Results The expression levels of LETM1 and HIF-1αin cancer tissues were higher than those in normal tissues adjacent to the cancer(P<0.05).There was a statistical significant difference in the maximum tumor diameter,lymph node metastasis,Ki-67,LETM1,and HIF-1αbetween the recurrence and non recurrence groups(P<0.05).According to logistic regression analysis,tumor maximum diameter over 5cm,Ki-67 positive,LETM1,and HIF-1αwere risk factors for recurrence in TNBC patients(OR>1,P<0.05).ROC curves were plotted to show that LETM1 and HIF-1α,both alone and in combination,had certain predictive value for recurrence in TNBC patients,with the highest predictive value observed in combination(P<0.05).Decision curve showed that within the threshold range of 0.12-0.95,the combined predic

关 键 词：三阴性乳腺癌 亮氨酸拉链EF-hand结构域跨膜蛋白1 缺氧诱导因子-1Α 预后 

分 类 号：R737.9[医药卫生—肿瘤]