膀胱尿路上皮癌中连接黏附分子A蛋白表达及临床意义  

The expression and clinical significance of JAM-A in bladder urothelial carcinoma

作　　者：赵英芳 隋贵春 王凤娇 洪海琪 赵冀山[1] 赵蓉[1] 范鑫月 李麟[1] 杨志刚[1] ZHAO Ying-fang;SUI Gui-chun;WANG Feng-jiao;HONG Hai-qi;ZHAO Ji-shan;ZHAO Rong;FAN Xin-yue;LI Lin;YANG Zhi-gang

机构地区：[1]包头市中心医院,内蒙古包头014040

出　　处：《中国疗养医学》2025年第3期102-106,共5页Chinese Journal of Convalescent Medicine

基　　金：内蒙古自治区包头市卫生健康科技计划项目资金资助(wsjkkj2022112)。

摘　　要：目的检测连接黏附分子A(junctional adhesion molecule-A,JAM-A)在膀胱尿路上皮癌(bladder urothelial carcinoma,BUC)中的表达状况,探讨JAM-A蛋白表达与尿路上皮癌发生发展以及临床病理指标的关系及意义。方法采用免疫组织化学SP法和Western blot方法检测了尿路上皮癌和癌旁组织中JAM-A表达状况,探讨JAM-A在各组间表达的差异及与临床病理特征之间的关系。结果免疫组织化学SP法结果显示20例癌旁组织中JAM-A表达只有3例(15%)阳性,而肿瘤组织中JAM-A表达阳性率为100%(119/119),JAM-A蛋白表达显著高于癌旁组织(P<0.001);Western blot结果JAM-A蛋白表达在尿路上皮癌组织表达量(4.23±0.68)显著高于癌旁组织(2.18±0.57),差异有高度统计学意义(P<0.001),相关性分析结果显示JAM-A蛋白表达与尿路上皮癌浸润、淋巴结转移相关;JAM-A蛋白高表达与尿路上皮癌浸润(P<0.05)、淋巴结转移(P<0.001)呈正相关,而与慢性炎症、性别、年龄均无相关性。ROC结果显示,JAM-A在尿路上皮癌病理诊断中AUC值为0.878,灵敏度为66.4%、特异度为85%。结论尿路上皮癌中JAM-A高表达对病理诊断有一定的价值;与JAM-A蛋白低表达相比较,JAM-A蛋白高表达更容易发生浸润和淋巴结转移,表明JAM-A蛋白高表达参与了尿路上皮癌的发生发展过程,可能对临床不良预后有一定的参考意义。Objective To detect the expression of junctional adhesion molecule-A(JAM-A)in bladder urothelial carcinoma(BUC),and to identify its clinical significance in the progression and clinicopathological parameters of BUC.Methods Expression levels of JAM-A in BUC and paracancerous specimens were detected by immunohistochemistry(SP method)and Western blot.Its correlation with clinicopathologic parameters was analyzed.Results Immunohistochemistry showed only 3 positive cases(15%)of JAM-A expression in 20 paracancerous specimens,but 100%(119/119)positivity for BUC.Protein level of JAM-A was significantly higher in the BUC specimens than paracancerous specimens(P<0.001).Western blot showed a significantly higher protein level of JAM-A in the BUC specimens than paracancerous specimens([4.23±0.68]VS[2.18±0.57],P<0.001).The correlation analysis results showed that the protein expression of JAM-A was significantly associated with BUC infiltration(P<0.05)and lymph node metastasis(P<0.001)in a positive correlation.It was not correlated with chronic inflammation,gender,and age.The receiver operating characteristic(ROC)results showed that the area under the curve(AUC)of JAM-A in the pathological diagnosis of BUC was 0.878,with a sensitivity of 66.4%and a specificity of 85%.Conclusion The high expression of JAM-A in BUC has certain value in pathological diagnosis.BUC with higher JAM-A level is more prone to infiltration and lymph node metastasis than the low level of JAM-A.It suggests that high-level JAM-A participates in the progression of BUC,indicating a poor prognosis.

关 键 词：膀胱肿瘤 尿路上皮癌 JAM-A蛋白 免疫组织化学 蛋白免疫印迹 

分 类 号：R737.14[医药卫生—肿瘤]