银杏酮酯GBE50促进兔面神经挤压伤恢复的实验研究  

Experimental study of GBE50 on facial nerve after being crushed in rabbits

作　　者：卢海彬[1] 张冰冰 于慧[1] 王玉娥 吴蜀江[1] 王秋旭 LU Hai-bin;ZHANG Bing-bing;YU Hui;WANG Yu-e;WU Shu-jiang;WANG Qiu-xu(Department of Oral and Maxillofacial Surgery,Zhongshan Hospital Affiliated to Dalian University.Dalian 116001,Liaoning Province;Zunyi Medical University.Zunyi 563000,Guizhou Province,China)

机构地区：[1]大连大学附属中山医院口腔颌面外科,辽宁大连116001 [2]遵义医科大学,贵州遵义563000

出　　处：《上海口腔医学》2025年第1期14-18,共5页Shanghai Journal of Stomatology

基　　金：国家重点研发计划资助项目(2023YFC2508200)。

摘　　要：目的:比较研究银杏叶提取物(Ginkgo biloba extract,GBE)银杏酮酯(GBE50)与甲钴胺对面神经挤压损伤的再生修复作用。方法:日本健康大白兔随机分为GBE50治疗组、甲钴胺治疗组、正常对照组和模型对照组4组,每组10只。治疗组和模型对照组均建立面神经上颊支挤压伤模型,正常对照组仅在面部切开但不挤压神经。2个治疗组分别给予GBE50和甲钴胺灌胃,2个对照组分别给予1 mL生理盐水灌胃。治疗后28天,观察胡须和口轮匝肌运动情况,神经电生理、组织形态学变化,进行图像分析,评估面神经再生和功能恢复情况。采用SPSS 22.0软件包对数据进行统计学分析。结果:模型对照组兔胡须和口轮匝肌运动恢复情况最差,GBE50治疗组较甲钴胺治疗组恢复情况好。GBE50组动作电位潜伏期较甲钴胺组低,且均显著低于对照组(P<0.05)。GBE50组动作电位振幅高于甲钴胺组,且均显著高于对照组(P<0.05)。图像分析显示,GBE50组有髓轴突数和面积高于甲钴胺组,且均显著优于对照组(P<0.05)。结论:GBE50对面神经损伤修复效果和速度优于甲钴胺,有望成为快速有效治疗面神经损伤的药物。PURPOSE:To compare the regeneration and repair effects of GBE50 and mecobalamin on facial nerve crush injury.METHODS:Forty healthy Japan giant ear rabbits were randomly divided into 4 groups,namely GBE50 treatment group,mecobalamin treatment group,normal control group and model control group.Each group contained 10 rabbits.Superior buccal branch of facial nerve cursh injured models were established for 2 treatment groups and model control group,while facial incision was made without crush injuries of facial nerve for normal control group.The treatment group was given GBE50 and mecobalamin respectively,while the control group was only given 1 mL of normal saline,respectively.After 28 days of treatment,rabbits'facial injured beard and orbicularis oris muscle movements were observed,neuroelectrophysiological tests,histomorphological observation,and image analysis were performed,facial nerve regeneration and functional recovery were detected.The data were analyzed by SPSS 22.0 software package.RESULTS:In the model control group,the beard and orbicularis oris muscle movement recovery of rabbits were the worst,but GBE50 treatment group showed better recovery than mecobalamin treatment group.The action potential latency in GBE50 group was lower than the mecobalamin group,and significantly lower than the control group(P<0.05).The action potential amplitude of GBE50 group was higher than that of mecobalamin group,and significantly higher than the control group(P<0.05).Image analysis result of never myelinated axons area and axon counts of GBE50 group was higher than that of mecobalamin group,and significantly higher than control group(P<0.05).CONCLUSIONS:GBE50 is superior to mecobalamin in efficacy and speed of facial nerve repair,and it is expected to become another therapeutic drug for rapid and effective treatment of facial nerve injury in clinical practice.

关 键 词：银杏酮酯 甲钴胺 面神经损伤 修复 

分 类 号：R741.05[医药卫生—神经病学与精神病学]