RNA m6A甲基化与肝细胞癌的相关性及对肝癌患者预后作用的Meta分析  

A Meta analysis of correlation of RNA m6A methylation to prognosis of patients with hepatocellular carcinoma

作　　者：何培 冯磊[1] 曹向红[1] 王佳[1] 彭传梅 孔凡斌[1] 陶春晖 张振华[2] He Pei;Feng Lei;Cao Xianghong(Clinical Laboratory,Yan'an Hospital Affiliated to Kunming Medical University,Kunming 650000,Yunan Province,China)

机构地区：[1]昆明医科大学附属延安医院检验科,昆明市650000 [2]安徽医科大学第二附属医院感染病科

出　　处：《实用肝脏病杂志》2025年第2期254-257,共4页Journal of Practical Hepatology

基　　金：国家自然科学基金地区科学基金资助项目(编号:82160402);云南省医学领军人才培养计划项目(编号:L-2019022);云南省科技厅-昆明医科大学应用基础研究联合专项重点项目(编号:202301AY070001-024);云南省教育厅-青年人才基础研究计划项目(编号:2024J0276)。

摘　　要：目的许多研究探讨了RNA m6A甲基化修饰器在肝细胞癌(HCC)中的作用,但目前的研究结果存在争议。因此,我们进行了荟萃分析以全面研究RNA m6A甲基化与HCC风险的关系以及对生存预后的影响。方法应用计算机检索2024年3月以前的中文数据库(知网和万方)、外文数据库(Pubmed、OVID、EBSCO、web of science)所收录的相关的中外文文献,收集有关RNA m6A甲基化与HCC发展、预后的关联文献病例对照研究。依照纽卡斯尔-渥太华量表(NOS)对纳入的中外文的文献行质量评价。应用RevMan 5.3软件行统计学分析。结果纳入文献11篇,累计病例3586例,其中HCC组1071例,癌旁组织组2515例;HCC组织m6A甲基化阳性率为61.7%,显著高于肝组织【38.5%,OR=4.77,95%CI(2.58~8.82),P<0.00001】;HCC组织FTO、METTL3、YTHDF1和ALKBH5表达量与患者预后密切相关【HR=1.3,95%CI(1.17~1.44),P<0.00001】。结论RNA m6A高甲基化与HCC发生密切相关,且HCC组织m6A甲基化修饰器FTO、METTL3、YTHDF1和ALKBH5高表达者预后差。Objective Many studies have explored the role of RNA m6A methylation modifiers in hepatocellular carcinoma(HCC),but the current results are controversial.Therefore,we conducted a meta-analysis to comprehensively study the relationship between RNA m6A methylation and the risk of liver cancer and the prognosis of patients with HCC.Methods We searched Chinese and English literatures database before March 2024,including Chinese databases Wanfang and CNKI,and English databases,Pubmed,OVID,EBSCO and web of science.Related literatures as case-control studies,on RNA m6A methylation and the development and prognosis of patients with HCC were retrieved.The quality of the literature was evaluated according to the Newcastle-Ottawa scale(NOS)scoring system.Based on inclusion and exclusion criteria,the literature was screened and the data was extracted,and the RevMan5.3 software was applied for statistical analysis.Results A total of 11 articles were found,including 3586 patients with HCC(1071 HCC tissues and 2515 adjacent liver tissue);the positive rate of m6A methylation in HCC tissues was 61.7%,significantly higher than 38.5%[OR=4.77,95%CI(2.58-8.82),P<0.00001]in liver tissues;hepatic expressions of FTO,METTL3,YTHDF1 and ALKBH5 were positively correlated to poor prognosis of patients with HCC[HR=1.3,95%CI(1.17-1.44),P<0.00001].Conclusion RNA m6A hypermethylation is closely related to the occurrence of liver cancer,and the expressions of m6A methylation modifiers,such as FTO,METTL3,YTHDF1 and ALKBH5 genes in liver cancer tissues are closely related to the poor prognosis of patients with HCC.

关 键 词：肝细胞癌 m6A RNA 甲基化 预后 荟萃分析 

分 类 号：R73[医药卫生—肿瘤]