长链非编码RNA LUCAT1调控胰腺癌MIA PaCa-2细胞恶性机制研究  

Study on the mechanism of long non-coding RNA LUCAT1 regulating the malignancy of pancreatic cancer MIA PaCa-2 cells

作　　者：叶梦洁 瞿薇薇 骆广涛 Ye Mengjie;Qu Weiwei;Luo Guangtao(Dept of Breast Surgery,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学附属第一医院乳腺外科,合肥230022

出　　处：《安徽医科大学学报》2025年第2期187-194,共8页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:82002534);安徽省转化医学研究院科研基金(编号:2022zhyx-C71)。

摘　　要：目的探究长链非编码RNA(lncRNA)LUCAT1对人胰腺癌细胞MIA PaCa-2生物学行为的影响,探讨LUCAT1在胰腺癌恶性进展中的潜在作用。方法在线数据库GEPIA分析LUCAT1在胰腺癌中的突变及表达情况;q-PCR实验检测对比人胰腺导管细胞HPNE及人胰腺癌细胞中LUCAT1的表达水平;FISH检测人胰腺癌组织中LUCAT1表达及分布。CCK-8实验和Transwell实验检测LUCAT1对MIA PaCa-2细胞增殖、凋亡、耐药及迁移能力的影响。基因集合富集分析比对LUCAT1参与的相关信号通路,Western blot实验验证蛋白质表达水平。结果GEPIA分析结果显示,LUCAT1在人胰腺癌组织中表达水平上调;q-PCR实验结果显示,人胰腺癌细胞中LUCAT1高表达,差异有统计学意义(P<0.05)。敲低及过表达LUCAT1可影响胰腺癌细胞增殖、凋亡、吉西他滨耐药性以及迁移和侵袭能力(P<0.05)。在胰腺癌中LUCAT1影响p-Akt表达水平,并在Akt抑制剂MK-2206处理后相关功能被抑制。结论LUCAT1通过PI3K-Akt信号通路调控胰腺癌细胞MIA PaCa-2恶性进展。Objective To investigate the effect of long non-coding RNA LUCAT1(lncRNA LUCAT1)on the biological behavior of MIA PaCa-2 in human pancreatic cancer cells,and to explore the potential role of LUCAT1 in the malignant progression of pancreatic cancer.Methods The mutation and expression of LUCAT1 in pancreatic cancer were analyzed by GEPIA,the expression levels of LUCAT1 in human pancreatic ductal cells HPNE and human pancreatic cancer cells were detected by q-PCR,and the expression and distribution of LUCAT1 in human pancreatic cancer tissues were detected by FISH.CCK-8 assay and Transwell assay were used to detect the effects of LUCAT1 on the proliferation,apoptosis,drug resistance,and migration of MIA PaCa-2 cells.Gene ensemble enrichment analysis was performed to compare the related signaling pathways involved in LUCAT1,and Western blot assay was used to verify the protein expression level.Results The results of GEPIA analysis showed that the expression level of LUCAT1 in human pancreatic cancer tissues was up-regulated,and the expression of LUCAT1 in human pancreatic cancer cells was significantly higher(P<0.05).Knockdown and overexpression of LUCAT1 could affect the proliferation,apoptosis,gemcitabine resistance,migration and invasion of pancreatic cancer cells,and the differences were statistically significant(P<0.05).In addition,LUCAT1 affected p-Akt expression levels in pancreatic cancer and was inhibited after treatment with Akt inhibitor MK-2206.Conclusion LUCAT1 regulates the malignant progression of MIA PaCa-2 in pancreatic cancer cells through the PI3K-Akt signaling pathway.

关 键 词：长链非编码RNA LUCAT1 胰腺癌 MIA PaCa-2 肿瘤耐药 AKT 

分 类 号：R735.9[医药卫生—肿瘤]