血管内皮生长因子B与成纤维生长因子在维持骨骼肌质量中的作用  

Role of vascular endothelial growth factor B and fibroblast growth factor in maintenance of skeletal muscle mass

作　　者：赵衍朴 杨小雨 于惠康 杨雪玲 杨春华 田梗 Zhao Yanpu;Yang Xiaoyu;Yu Huikang;Yang Xueling;Yang Chunhua;Tian Geng(Shandong Technology Innovation Center of Molecular Targeting and Intelligent Diagnosis and Treatment,College of Pharmacy,Binzhou Medical University,Yantai 264000)

机构地区：[1]滨州医学院药学院,山东省分子靶向智能诊疗技术创新中心,烟台264000

出　　处：《安徽医科大学学报》2025年第2期293-299,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:31771284);山东省泰山学者项目(编号:tstp20221145)。

摘　　要：目的研究正常和高脂饮食情况下血管内皮生长因子B(VEGFB)在维持骨骼肌质量中的作用,并探讨VEGFB与成纤维生长因子(FGF)信号通路之间的串扰在这一过程中发挥的作用。方法设置4个实验组:正常饮食VEGFB^(+/+)及VEGFB^(-/-)组,高脂饮食VEGFB^(+/+)及VEGFB^(-/-)组;获取24周龄小鼠骨骼肌,称重;采用基因表达关联分析和qPCR实验探究骨骼肌中FGFs的表达水平。结果两种饮食条件下VEGFB缺失均会导致小鼠骨骼肌质量下降;正常饮食条件下,VEGFB^(-/-)小鼠骨骼肌中8个FGFs表达水平下调,其中6个为旁分泌FGFs;高脂饮食条件下,VEGFB^(-/-)小鼠骨骼肌中11个FGFs表达水平下调,其中8个为旁分泌FGFs。结论骨骼肌中VEGFB可通过FGFs网络参与维持骨骼肌质量。Objective To verify the role of vascular endothelial growth factor B(VEGFB)in maintenance of skeletal muscle mass under chow-fed and high-fat diet,and to investigate the role of crosstalk between VEGFB and fibroblast growth factor(FGF)signaling pathways in the process.Methods Four experimental groups were designed:VEGFB^(+/+)chow-fed diet group,VEGFB^(-/-)chow-fed diet group,VEGFB^(+/+)high-fat diet group,VEGFB^(-/-)high-fat diet group.Skeletal muscles from 24 weeks mice were isolated and weighed.Gene expression association analysis and qPCR experiments were conducted to assess FGFs expression levels.Results Under both dietary conditions,VEGFB ablation resulted in reduced muscle mass.Under chow-fed diet condition,8 FGFs level reduced including 6 paracrine FGFs in the skeletal muscle from VEGFB^(-/-)mice.Under high-fat diet condition,11 FGFs level decreased including 8 paracrine FGFs in VEGFB^(-/-)mice.Conclusion VEGFB may participate in regulating skeletal muscle mass through FGF networks in the skeletal muscles.

关 键 词：骨骼肌 血管内皮生长因子B 成纤维生长因子 基因敲除小鼠 高脂饮食 FGF6 

分 类 号：Q593.3[生物学—生物化学]