小麦蛋白质二硫键异构酶基因家族的鉴定与表达  

Identification and Expression Analysis of Protein Disulfide Isomerase Gene Family in Wheat

作　　者：葛仕杰 刘怡德 张华东 宁强 朱展望[1] 王书平[2] 刘易科[1,2] GE Shi-jie;LIU Yi-de;ZHANG Hua-dong;NING Qiang;ZHU Zhan-wang;WANG Shu-ping;LIU Yi-ke(Institute of Food Crops,Hubei Academy of Agricultural Sciences,Key Laboratory of Crop Molecular Breeding,Ministry of Agriculture and Ru-ral Affairs,Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement,Wuhan 430064;MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtza River(Co-Construction by Ministry and Province),College of Agronomy,Yangtze Univer-sity,Jingzhou 434025)

机构地区：[1]湖北省农业科学院粮食作物研究所农业农村部作物分子育种重点实验室粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064 [2]长江大学农学院农业农村部长江中游作物绿色高效生产重点实验室(部省共建),荆州434025

出　　处：《生物技术通报》2025年第2期85-96,共12页Biotechnology Bulletin

基　　金：国家自然科学基金项目(32272170);武汉市知识创新专项(2022020801010341)。

摘　　要：【目的】通过对小麦蛋白质二硫键异构酶PDIL基因家族成员进行鉴定及表达分析,分析其在小麦不同器官和不同发育时期以及响应生物和非生物胁迫中的表达模式,为明确小麦PDIL基因家族功能奠定基础。【方法】以模式植物拟南芥和禾本科作物大麦PDIL基因家族蛋白序列为诱饵,在基因组数据库对小麦PDIL家族基因进行全基因组鉴定,利用生物信息学方法对其基因的理化性质、系统进化、染色体位置及基因结构进行分析,并分析PDILs基因在小麦生长发育、生物及非生物逆境的表达模式。【结果】鉴定得到31个小麦PDIL基因家族成员均为亲水蛋白,其中26个为稳定蛋白;亚细胞定位预测表明,小麦PDIL蛋白主要分布在细胞质和叶绿体中,在内质网和细胞核中也有分布;小麦PDIL基因家族可分为7个进化分支,同一分支基因结构和保守基序相似或相同,非均匀分布在19个染色体上;小麦PDIL基因家族成员含有多种响应逆境胁迫以及与水杨酸(SA)、茉莉酸甲酯(MeJA)、赤霉素(GA)、脱落酸(ABA)和生长素(IAA)等5类植物激素相关的顺式作用元件,部分基因具有与昼夜节律控制以及种子特性调控相关的元件;转录组数据表明,TaPDIL4-1A、TaPDIL4-1B和TaPDIL4-1D在不同生长时期组织都有较高的表达量;转录组分析和RT-PCR结果显示,TaPDIL4-1B在禾谷镰刀菌侵染后相对表达量升高,TaPDIL1-4A在干旱胁迫下表达量提高。【结论】31个小麦PDIL基因家族成员在进化过程中具有保守性,不仅参与小麦品质形成,还广泛参与生物和非生物逆境调控,其中TaPDIL4-1B可能参与小麦赤霉病抗性的调控,TaPDIL1-4A可能参与小麦干旱胁迫的应答。【Objective】The objective of this study is to perform the genome-wide identification of the wheat PDIL(Protein Disulfide Isomerase)gene family members,and to analyze their expression patterns in different organs and developmental stages of wheat,as well as in response to biotic and abiotic stress,which lays the foundation for clarifying the function of wheat PDIL gene family.【Method】Using protein sequences of PDIL gene family of Arabidopsis thaliana and Gramineae crop barley as bait,genome-wide identification of wheat PDIL family genes was conducted in the genome database.Then the physicochemical properties,phylogenetics,chromosome position and gene structure were analyzed using bioinformatics methods.And the expression pattern of PDILs genes in wheat growth and development,biotic and abiotic adversity were analyzed.【Result】The 31 wheat PDIL gene family members were identified as hydrophilic proteins,of which 26 were stable proteins.Subcellular localization prediction indicated that wheat PDIL proteins were mainly distributed in the cytoplasm and chloroplasts,as well as in the endoplasmic reticulum and nucleus.The wheat PDIL gene family could be divided into 7 evolutionary branches,and the gene structure and conserved motif of the same branch were similar or identical,and they were not uniformly distributed on 19 chromosomes.The promoters of wheat PDIL gene family members contained multiple cis-acting elements that responded to stress and associated with five types of plant hormones,including salicylic acid(SA),methyl jasmonate(MeJA),gibberellin(GA),abscisic acid(ABA),and auxin(IAA),and a few genes also contained elements related to circadian rhythm control and seed trait regulation.Transcriptome data showed that TaPDIL4-1A,TaPDIL4-1B,and TaPDIL4-1D genes had high expressions in tissues at different growth stages.Transcriptome analysis and RT-PCR results showed that the relative expression of TaPDIL4-1B increased after infection with Fusarium graminearum,and the expression of TaPDIL1-4A gene increased

关 键 词：小麦 PDIL基因家族 表达分析 生物胁迫 非生物胁迫 

分 类 号：S51[农业科学—作物学]