Dual roles ofα1,4-galactosyltransferase 1 in spermatogenesis of Drosophila melanogaster  

作　　者：Yanhong Xiao Bo Huang Sibo Chen Zhikai Lin Zhiying Zhu Yuzhen Lu Xiao-Qiang Yu Liang Wen Qihao Hu 

机构地区：[1]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Guangzhou Key Laboratory of Insect Development Regulation and Application Research,Institute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,China [2]School of Life Sciences,Hubei Key Laboratory of Genetic Regulation and Integrative Biology,Central China Normal University,Wuhan,China

出　　处：《Insect Science》2025年第1期127-139,共13页昆虫科学(英文版)

基　　金：supported by the National Natural Science Foundation of China(32300389);Guangdong Basic and Applied Basic Research(2022A1515111055).

摘　　要：Spermatogenesis is critical for insect reproduction and the process is regulated by multiple genes.Glycosyltransferases have been shown to participate in the development of Drosophila melanogaster;however,their role in spermatogenesis is still unclear.In this study,we found thatα1,4-galactosyltransferase 1(α4GT1)was expressed at a significantly higher level in the testis than in the ovary of Drosophila.Importantly,the hatching rate was significantly decreased whenα4GT1 RNA interference(RNAi)males were crossed with w1118 females,with only a few mature sperm being present in the seminal vesicle ofα4GT1 RNAi flies.Immunofluorescence staining further revealed that the individualization complex(IC)in the testes fromα4GT1 RNAi flies was scattered and did not move synchronically,compared with the clustered IC observed in the control flies.Terminal deoxyribonucleotide transferase(TdT)-mediated dUTP nick end labeling(TUNEL)assay showed that apoptosis signals in the sperm bundles ofα4GT1 RNAi flies were significantly increased.Moreover,the expression of several individualization-related genes,such as Shrub,Obp44a and Hanabi,was significantly decreased,whereas the expression of several apoptosis-related genes,including Dronc and Drice,was significantly increased in the testes ofα4GT1 RNAi flies.Together,these results suggest thatα4GT1 may play dual roles in Drosophila spermatogenesis by regulating the sperm individualization process and maintaining the survival of sperm bundles.

关 键 词：APOPTOSIS α4GT1 GALACTOSYLTRANSFERASE INDIVIDUALIZATION SPERMATOGENESIS 

分 类 号：R73[医药卫生—肿瘤]