精苓口服液对注意缺陷多动障碍动物模型大鼠记忆功能的作用研究  

Effect of Jingling oral liquid on memory function in rats of ADHD animal model

作　　者：刘力维 程申 杨育访 陈健[1] 陈玉燕[1] LIU Liwei;CHENG Shen;YANG Yufang;CHEN Jian;CHEN Yuyan(The First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310000,China)

机构地区：[1]浙江中医药大学附属第一医院儿科,杭州310000

出　　处：《中国中西医结合儿科学》2025年第1期27-32,F0003,共7页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：浙江省中医药科技计划项目中医药传承创新人才支持计划(2024ZR014);吴阶平医学基金会临床科研专项资助基金。

摘　　要：目的探究精苓口服液对注意缺陷多动障碍(ADHD)动物模型幼龄自发性高血压大鼠记忆功能改善作用及促进髓鞘发育作用。方法24只自发性高血压大鼠随机分为模型组、托莫西汀组和精苓口服液等剂量[11.15 g/(kg·d)]、高剂量组[29.5 g/(kg·d)],每组6只,另设Wistar大鼠6只为正常对照组。治疗6周后水迷宫观察大鼠工作记忆改善情况。分离各组大鼠前额叶,固蓝染色观察神经组织的髓鞘结构。Western blot测定大鼠前额叶Nogo-A、髓磷脂调节因子(MRF)、成纤维细胞生长因子17(FGF17)、神经元胶质抗原2(NG2)、髓鞘碱性蛋白质(MBP)表达情况。结果(1)治疗6周后,与托莫西汀组比较,精苓口服液等剂量及高剂量组可较稳定促进大鼠体质量增长。(2)与模型组相比,托莫西汀组、精苓口服液等剂量及高剂量组治疗6周后自发性高血压大鼠水迷宫找台时间缩短。(3)与模型组相比,精苓口服液各剂量组脑组织髓鞘排列紧密、结构完整。(4)与模型组相比,精苓口服液等剂量组和高剂量组NG2及MRF蛋白表达均升高,高剂量组MBP表达升高,等剂量组FGF17表达降低,差异均有统计学意义(P<0.05)。结论精苓口服液可促进ADHD模型自发性高血压大鼠髓鞘新生成熟,改善自发性高血压大鼠记忆学习能力。Objective To explore the effect of Jingling oral liquid on improving memory function and promoting myelination in young spontaneously hypertensive rats(SHR)of ADHD animal model.Methods A total of 24 SHR were randomly divided into model group,tomoxetine group,Jingling oral liquid equal-dose group[11.15 g/(kg·d)]and high-dose group[29.5 g/(kg·d)],with 6 rats in each group,and 6 Wistar Kyoto rats(WKY)were included as control group.The improvement in working memory of the rats was observed by water maze after 6 weeks of treatment.The prefrontal lobes of the rats in each group were separated,and the structures of myelin sheath were observed by luxol fast blue(LFB).The expressions of Nogo-A,MRF,FGF17,NG2 and MBP in the frontal lobe of rats were determined by western blot.Results(1)After 6 weeks of treatment,compared with the tomoxetine group,both the Jingling oral liquid equal-dose group and high-dose group demonstrated a more stable promotion of body weight gain in rats.(2)Compared with the model group,the finding time in water maze of SHR in tomoxetine group and Jingling equal-dose and high-dose groups was shortened after 6 weeks of treatment.(3)Compared with model group,myelin in brain tissues of rats in equal-dose and high-dose groups was tightly arranged with intact structure(P<0.05).(4)Compared with model group,NG2 and MRF protein expressions were increased in both Jingling oral liquid equal-dose group and high-dose group,MBP expression was elevated in the high-dose group,while the FGF17 expression decreased in the equal dose-group,with all differences being statistically significant(P<0.05).Conclusion Jingling oral liquid can improve the memory and learning ability of SHR,and promote myelin regeneration and maturation.

关 键 词：注意缺陷多动障碍 工作记忆 精苓口服液 髓鞘发育 大鼠 

分 类 号：R749.93[医药卫生—神经病学与精神病学]