Transcription Factors and Retained Intron Act Vital Roles in Cadmium Stress Response of Medicinal Model Plant Salvia miltiorrhiza  

作　　者：Jun Yuan Rongpeng Liu Xiaoyun Wang Haihui Fu 

机构地区：[1]College of Traditional Chinese Medicine(College of Life Sciences),Jiangxi University of Chinese Medicine,Nanchang,330004,China [2]School of Agricultural Sciences,Jiangxi Agricultural University,Nanchang,330045,China [3]Research Center for Traditional Chinese Medicine Resources and Ethnic Minority Medicine,Jiangxi University of Chinese Medicine,Nanchang,330004,China [4]School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang,330004,China

出　　处：《Phyton-International Journal of Experimental Botany》2024年第9期2267-2284,共18页国际实验植物学杂志(英文)

基　　金：supported by Jiangxi Provincial Natural Science Foundation(20232BAB216119);Science and Technology Research Project of Jiangxi Provincial Department of Education(GJJ2200978);Jiangxi Provincial Health Commission S&T Plan(SKJP220227132);Jiangxi Provincial Administration of Traditional Chinese Medicine S&T Program(2022A392).

摘　　要：Cadmium(Cd)has seriously affected the quality of traditional Chinese medicinal material Salvia miltiorrhiza in recent years,threatening human health.The physiological and metabolic profiles of S.miltiorrhiza in response to Cd stress have been revealed in previous studies.However,transcriptional and post-transcriptional regulation in response to different degrees of Cd(0,25,50,and 100 mg/kg)stress in S.miltiorrhiza remains unclear.Here,transcriptome atlas in S.miltiorrhiza under different degrees of Cd Stress was unveiled using RNA sequencing(RNA-seq).These results showed that the profiles of gene expression were different in the response to Cd treatment.Defense response-related biological processes were involved in differentially expressed genes(DEGs).In total,1966 genes were identified as transcription factors(TFs)with seven expressed trends.Retained intron(RI)was the major phenomenon.Targeted genes of intron splicing factors were identified via weighted gene co-expression network analysis(WGCNA).All of these indicated that transcriptional and post-transcriptional regulations were involved in response to Cd stress in S.miltiorrhiza.Our study will provide the most comprehensive resource for studying heavy metals in traditional Chinese medicine plants.

关 键 词：Cd stress differentially expressed genes retained intron Salvia miltiorrhiza transcription factors 

分 类 号：R73[医药卫生—肿瘤]