基于桔梗皂苷D检测的HPLC-CAD试验设计  

Experimental Design of HPLC-CAD Based on Detection of Platycodon Saponin D

作　　者：司晓晶 冯华峰 陈旭[2] 张娟[2] SI Xiaojing;FENG Huafeng;CHEN Xu;ZHANG Juan(School of Food Science,Shanghai Business University,Shanghai 200235;School of Life Sciences,Shanghai University,Shanghai 200444)

机构地区：[1]上海商学院食品系,上海200235 [2]上海大学生命科学学院,上海200444

出　　处：《食品工业》2025年第1期322-324,共3页The Food Industry

基　　金：上海市教育委员会2023年度市级重点课程建设项目现代仪器分析、生物仪器分析;上海商学院一流本科课程建设项目-现代仪器分析、食品安全监督管理。

摘　　要：建立测定桔梗原料中桔梗皂苷D含量的高效液相色谱-电雾式检测器(HPLC-CAD)仪器分析试验设计。基于Thermo Scientific^(TM)Corona^(TM)Veo^(TM)RS CAD检测器,以中药原料桔梗为例,建立桔梗皂苷D的高效液相色谱法,并对方法的可行性、可靠性和适用性进行分析。结果表明,桔梗皂苷D在0.5~50μg/mL之间进样量浓度与峰面积对数呈良好线性关系A=0.0397C+0.0654(R^(2)=0.9926),样品稀释液浓度为1.70μg/mL,平均加样回收率为112.8%(SRSD=1.9%)。试验设计可靠、准确,具有良好的重复性,为桔梗原料的质量标准提升奠定基础,并能激发学生的科研兴趣,提高学生的科研素养和创新能力。Establish an experimental design for the determination of platycodon saponin D in platycodonplatycodon raw materials by high performance liquid chromatography with charged aerosol detector(HPLC-CAD).A method is established based on Thermo Scientific^(TM)Corona^(TM)Veo^(TM)RS CAD detector and high performance liquid chromatography(HPLC)for the determination of platycodonplatycodon saponin D from platycodongrandiflorus.The feasibility,reliability and applicability of the method are analyzed.The results show that the concentration of platycodon D in the range of 0.5-50μg/mL shows a good linear relationship with the peak area,A=0.0397C+0.0654(R^(2)=0.9926),and the concentration of sample diluent is 1.70μg/m L.The average recovery is 112.8%(SRSD=1.9%).The experimental design is reliable,accurate and repeatable.It can not only lay a foundation for the improvement of the quality standard of platycodon grandidon raw materials,but also stimulate students'interest in scientific research and improve their scientific research literacy and innovation ability.

关 键 词：桔梗皂苷D 高效液相色谱-电喷雾散射检测器(HPLC-CAD) 含量测定 仪器分析试验 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学] TS207.3[理学—分析化学]