过表达OsTTG1影响拟南芥的表皮毛、花青素及开花期  

Overexpression of OsTTG1 affects the epidermal trichome,anthocyanins,and flowering stage in Arabidopsis thaliana(L.)Heynh.

作　　者：罗双双 张宗琼[2] 农保选[2,3] 夏秀忠 梁树辉[2] 廖祖宇 冯锐 陈灿[2] 郭辉[2] 梁云涛[2] 曾宇[2] 邱永福[1,3] 李丹婷[2,3] 杨行海 Luo Shuangshuang;Zhang Zongqiong;Nong Baoxuan;Xia Xiuzhong;Liang Shuhui;Liao Zuyu;Feng Rui;Chen Can;Guo Hui;Liang Yuntao;Zeng Yu;Qiu Yongfu;Li Danting;Yang Xinghai(College of Agriculture,Guangxi University,Nanning 530004,China;Rice Research Institute,Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding,Nanning 530007,China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,Nanning 530004,China)

机构地区：[1]广西大学农学院,南宁530004 [2]广西农业科学院水稻研究所/广西水稻遗传育种重点实验室,南宁530007 [3]亚热带农业生物资源保护与利用国家重点实验室,南宁530004

出　　处：《植物科学学报》2025年第1期82-91,共10页Plant Science Journal

基　　金：广西重点研发计划项目(桂科AB21238009);国家自然科学基金项目(32060476,32260487,32360519);广西重大科技创新基地项目(2022-36-Z01-ZZ07)。

摘　　要：OsTTG1编码WD40结构域重复蛋白,在水稻(Oryza sativa L.)中与花青素积累有关,但是否影响其他性状尚不清楚。本研究中,我们在拟南芥(Arabidopsis thaliana(L.)Heynh.)突变体ttg1中过表达OsTTG1基因,结果显示,OsTTG1-OE株系的叶片和茎秆上均有花青素积累,种子呈棕褐色,叶片、茎秆、花萼等部位均出现表皮毛。突变体ttg1的开花时间比Col-0和OsTTG1-OE株系早了大约3~5 d,而Col-0和OsTTG1-OE的开花时间没有明显差异。利用RNA-seq技术分析OsTTG1-OE与ttg1在不同时期的差异表达基因(DEGs),发现这些DEGs包括可能与花青素合成相关的基因(MKK9、CYP75B1、TT8、GSTF12、DFR、F3H、CHS、LDOX等)、可能与表皮毛形成相关的基因(SPL15、TCL1、ETC2、SPL4、SPL13A、ETC3、GIS等)以及可能与开花相关的基因(FLC、FT、AGL19、HDA05、LHY等)。研究结果可为了解OsTTG1在水稻中的功能提供基础和借鉴。The OsTTG1 gene encodes a WD40 domain repeat protein,with its knockout in Oryza sativa L.known to affect anthocyanin accumulation.However,whether this gene affects other traits remains unclear.In this study,OsTTG1 was overexpressed in the Arabidopsis thaliana(L.)Heynh.ttg1 mutant.Results showed that overexpression of OsTTG1(OsTTG1-OE)restored anthocyanin accumulation in leaves and stalks,produced dark brown seeds,and promoted epidermal hair development on leaves,stalks,and calyces.In contrast,the ttg1 mutant exhibited flowering 3–5 d earlier than both the Col-0 and OsTTG1-OE strains,while no significant differences in flowering time were observed between Col-0 and OsTTG1-OE.RNA-seq analysis of differentially expressed genes(DEGs)between OsTTG1-OE and ttg1 at various developmental stages revealed key gene clusters potentially associated with anthocyanin synthesis(MKK9,CYP75B1,TT8,GSTF12,DFR,F3H,CHS,and LDOX),epidermal hair formation(SPL15,TCL1,ETC2,SPL4,SPL13A,ETC3,and GIS),and flowering regulation(FLC,FT,AGL19,HDA5,and LHY).These findings provide an important theoretical basis and reference for understanding the function of OsTTG1 in O.sativa.

关 键 词：OsTTG1 拟南芥 过表达 表皮毛 花青素 开花期 

分 类 号：Q943.2[生物学—植物学]