CD147通过MAPK信号通路影响宫颈癌细胞的生物学功能  

CD147 affects the biological function of cervical cancer cells through MAPK signaling pathway

作　　者：尚香玉 李金秋 严怡然 阿仙姑·哈斯木 SHANG Xiangyu;LI Jinqiu;YAN Yiran;ASHAMGUL Hasim(Xinjiang Key Laboratory of Molecular Biology of Endemic Diseases,Basic Medicine College of Xinjiang Medical University,Urumchi 830054,China)

机构地区：[1]新疆医科大学基础医学院,新疆地方病分子生物学实验室,乌鲁木齐830054

出　　处：《生命的化学》2025年第1期152-159,共8页Chemistry of Life

基　　金：国家自然科学基金项目(82260516)。

摘　　要：本文探讨了下调人分化簇147(human cluster of differentiation 147,CD147)对子宫颈癌SiHa细胞系侵袭、迁移、增殖和凋亡的影响及潜在机制。采用TNPLOT和HPA数据库分析CD147在人子宫颈癌组织和正常的子宫颈组织中mRNA、蛋白表达差异。实时荧光定量聚合酶链式反应(real-time fluorescent quantitative polymerase chain reaction,qRT-PCR)和Western blot方法检测宫颈癌细胞及正常宫颈上皮细胞中CD147的mRNA、蛋白表达情况。利用慢病毒转染技术构建CD147敲降稳定转染细胞株,qRTPCR和Western blot及荧光观察确定转染效率及敲降效果。进一步采用Western blot检测E-cadherin、Ncadherin、丝裂原活化蛋白激酶(mitogen activated protein kinase,MAPK)、磷酸化丝裂原活化蛋白激酶(phosphorylated mitogen-activated protein kinase,P-MAPK)的表达水平;细胞划痕和Transwell实验分析细胞迁移、侵袭的能力;克隆形成实验评估细胞集落形成的能力;流式细胞仪检测细胞凋亡。结果发现,在TNPLOT和HPA数据库中人子宫颈癌组织CD147的mRNA和蛋白水平都显著高于正常子宫颈组织。qRT-PCR和Western blot结果显示,子宫颈癌细胞MS751、SiHa中CD147 mRNA和蛋白表达水平显著高于正常宫颈上皮细胞HCerEpiC,且SiHa细胞中的表达最高。转染慢病毒后,与阴性对照组相比,sh-CD147组中CD147 mRNA和蛋白表达水平明显降低;sh-CD147组细胞迁移、侵袭能力明显降低,细胞集落形成能力降低,而细胞凋亡率有所升高。此外,与阴性对照组相比,sh-CD147组中E-cadherin蛋白表达水平明显升高,而N-cadherin、P-MAPK蛋白表达水平显著降低。综上所述,下调CD147表达抑制宫颈癌SiHa细胞系迁移、侵袭及增殖能力并促进其凋亡,可能与抑制MAPK信号通路相关。The present study aimed to investigate the impact of down-regulated human cluster of differentiation 147(CD147)on invasion,migration,proliferation,and apoptosis in the SiHa cell line of cervical cancer,as well as its underlying mechanism.The mRNA and protein expression differences of CD147in cervical cancer tissues and normal cervical tissues were analyzed by TNPLOT and HPA database.The mRNA and protein expression levels of CD147 in cervical cancer cells and normal cervical epithelial cells were assessed through qRT-PCR and Western blot techniques.Lentivirus transfection technique was used to construct CD147 knockdown stable transfection cell line,q RT-PCR,Western blot and fluoresence observatiion were used to determine the transfection efficiency and knockdown effect.The expression levels of E-cadherin,N-cadherin,mitogen-activated protein kinase(MAPK)and phosphorylated mitogen-activated protein kinase(P-MAPK)were detected by Western blot.The ability of cell migration and invasion was analyzed by cell scratch and Transwell assay.Clonal formation assay was used to evaluate the ability of cell colony formation.Apoptosis was detected by flow cytometry.The results demonstrated a significant upregulation of CD147mRNA and protein levels in cervical cancer tissues compared to normal cervical tissues,as evidenced by the TNPLOT and HPA databases.Furthermore,the results of q RT-PCR and Western blot revealed a substantial increase in CD147 expression at both mRNA and protein levels in cervical cancer cells MS751 and SiHa when compared to normal cervical epithelial cells HCerEpiC,with the highest expression observed in SiHa cells.After transfection with lentivirus,the expression levels of CD147 mRNA and protein in sh-CD147 group were significantly decreased compared with negative control group.In sh-CD147 group,the ability of cell migration and invasion was decreased,the ability of cell colony formation was decreased,and the apoptosis rate was increased.In addition,compared with the negative control group,the expression leve

关 键 词：子宫颈癌 人分化簇147 丝裂原活化蛋白激酶 

分 类 号：R737.33[医药卫生—肿瘤]