溃疡性结肠炎患者相关环状RNA差异表达谱分析及功能研究  

Differential expression profile and functional study of related circular RNA in patients with ulcerative colitis

作　　者：董晓斌 张静 苏莎莎 莎比亚·沙吾提 盛好 Xiaobin Dong;Jing Zhang;Shasha Su;Hao Sheng(Department of Gastroenterology,the Fourth Clinical Medical College of Xinjiang Medical University,Urumqi 830000,China;Department of Gastroenterology,Xinjiang Uygur Autonomous Region Hospital of Traditional Chinese Medicine,Urumqi 830000,China)

机构地区：[1]新疆医科大学第四临床医学院消化内科,乌鲁木齐830000 [2]新疆维吾尔自治区中医医院消化内科,乌鲁木齐830000

出　　处：《中华结直肠疾病电子杂志》2024年第6期499-509,共11页Chinese Journal of Colorectal Diseases(Electronic Edition)

基　　金：新疆维吾尔自治区面上项目(No.2022D01C163)。

摘　　要：目的利用高通量测序和生物信息学技术,分析溃疡性结肠炎(UC)中环状RNA(circRNA)表达特征。方法选取医院确诊的8例UC患者为观察组,根据其病情和结肠镜下黏膜表现分为活动期组和缓解期组,每组各4例,另选4名健康体检者纳入对照组,运用circRNA高通量测序检测三组结肠组织中circRNAs和信使核糖核酸(mRNAs)表达谱,运用在线生物信息学软件关联分析差异circRNAs及预测的靶向微小核糖核酸(miRNAs)的生物学功能。结果高通量测序结果预处理分析中,三个样本组在总体基因的表达一致,通过对circRNAs和mRNAs的数据进行聚类分析发现,在三组样本中,circRNAs和mRNAs的基因表达存在明显的差异。筛选表达的circRNAs差异倍数≥1.5或≤0.5,其中活动期组同对照组比对差异circRNAs共217个,有135个表达上调,82个表达下调;缓解期组同对照组比对差异circRNAs共212个,有98个表达上调,114个表达下调;活动期组同缓解期组比对差异circRNAs共147个,有95个表达上调,52个表达下调,差异表达的circRNAs主要在免疫系统、炎症通路、细胞周期和信号转导通路的调控等功能上显著富集;运用miranda和RNAhybrid两种方法的交集预测7个免疫和炎症相关差异miRNA-circRNA靶标关系,分析circRNA-miRNA-UC分子调控机制。结论UC活动期和缓解期患者及健康者的结肠组织之间存在差异表达的circRNAs,且可能参与UC的致病调控过程。Objective To analyze the expression characteristics of circular RNA(circRNA)in ulcerative colitis(UC)using high-throughput sequencing and bioinformatics techniques.Methods Eight diagnosed UC patients from the hospital were selected as the observation group,divided into an active phase group and a remission phase group based on their condition and colonoscopic mucosal findings,with 4 cases in each group.Additionally,four healthy individuals were included in the control group.CircRNA highthroughput sequencing was used to detect the expression profiles of circRNAs and messenger RNAs(mRNAs)in the colon tissues of the three groups.Online bioinformatics software was utilized to correlate the biological functions of differentially expressed circRNAs and the predicted target microRNAs(miRNAs)of circRNAs.Results The preprocessing analysis of high-throughput sequencing results indicated that the overall gene expression was consistent among the three sample groups.Clustering analysis of circRNAs and mRNAs data revealed significant differences in gene expression among the three sample groups.CircRNAs with a fold-change of≥1.5 or≤0.5 were screened.A total of 217 differentially expressed circRNAs were identified between active phase group and Control group,with 135 upregulated and 82 downregulated;212 differentially expressed circRNAs between remission group and control group,with 98 upregulated and 114 downregulated;and 147 differentially expressed circRNAs between active phase group and remission group,with 95 upregulated and 52 downregulated.The differentially expressed circRNAs were significantly enriched in functions related to the immune system,inflammatory pathways,cell cycle,and signaling pathways.The intersection of miranda and RNAhybrid was used to predict 7 differential miRNA-circRNA target relationships related to immunity and inflammation,and the molecular regulatory mechanism of circRNAmiRNA-UC was analyzed.Conclusion There are differentially expressed circRNAs between the colon tissues of UC patients during

关 键 词：溃疡性结肠炎(UC) 环状RNA(CircRNA) 差异表达谱 

分 类 号：R73[医药卫生—肿瘤]