固相微萃取气相色谱-四极杆静电场轨道阱高分辨质谱法检测葡萄酒中53种挥发性有机物  

Detection of 53 Volatile Organic Compounds in Wine by Solid-Phase Microextraction Coupled with Gas Chromatography-Quadrupole Orbitrap High-Resolution Mass Spectrometry

作　　者：孟平 麦晨 郑思珩 赵勇 周衡刚 黎晔晖 张艳超 蒋小良 MENG Ping;MAI Chen;ZHENG Si-Heng;ZHAO Yong;ZHOU Heng-Gang;LI Ye-Hui;ZHANG Yan-Chao;JIANG Xiao-Liang(Huangpu Customs Technical Center,Guangzhou 510700;Guangdong Provincial Key Laboratory of Intelligent Port Security Inspection,Huangpu Customs District P.R.China,Guangzhou 510700;Guangzhou Baiyun Airport Customs Comprehensive Technical Service Center,Guangzhou 510470;Jiangmen Customs Technical Center,Jiangmen 529000)

机构地区：[1]黄埔海关技术中心,广州510700 [2]广东省口岸安全智能化检测重点实验室,广州510700 [3]广州白云机场海关综合技术服务中心,广州510470 [4]江门海关技术中心,江门529000

出　　处：《中国口岸科学技术》2025年第1期62-69,共8页China Port Science and Technology

基　　金：广东省口岸安全智能化检测重点实验室(2023B1212010011)。

摘　　要：本研究采用固相微萃取(Solid-Phase Microextraction,SPME)进样方式,气相色谱-四极杆/静电场轨道阱高分辨质谱(Gas Chromatography-Quadrupole/Orbitrap High-Resolution Mass Spectrometry,GC-Q/Qrbitrap HRMS)方法检测葡萄酒中53种挥发性有机物(Volatile Organic Compounds,VOCs)。样品经固相微萃取提取,仪器分析后利用仪器自带解卷积软件实现快速识别各种挥发性有机物。结果显示,方法检出限最低可达到0.01μg/L,在0.2~30μg/L范围内线性相关系数(R^(2))均在0.990以上;在不同添加水平下回收率在50%~130%之间。该方法前处理简单,适用于葡萄酒中痕量53种挥发性有机物的快速筛查及定量检测。This study employs solid-phase microextraction(SPME)for sample introduction and gas chromatographyquadrupole/orbitrap high-resolution mass spectrometry(GC-Q/Orbitrap HRMS)for the detection of 53 volatile organic compounds(VOCs)in wine.After extraction by SPME,the instrument’s built-in deconvolution software is utilized for rapid identification of various VOCs.The results show that the method’s detection limit can reach as low as 0.01μg/L,with a linear correlation coefficient(R^(2))above 0.990 in the range of 0.2-30μg/L;recoveries at different spiking levels are between 50%and 130%.This method is simple in sample preparation and is suitable for rapid screening and quantitative detection of trace 53 VOCs in wine.

关 键 词：气相色谱-四极杆/静电场轨道阱高分辨质谱 挥发性有机物 顶空 固相微萃取 

分 类 号：O65[理学—分析化学]