梓醇联合罗格列酮调节HMGB1/TLR4/NF-κB信号通路对多囊卵巢综合征大鼠胰岛素抵抗及炎症的改善作用  

Effects of catalpol combined with rosiglitazone on improving insulin resistance and inflammation in rats with polycystic ovary syndrome by modulating HMGB1/TLR4/NF-κB signaling pathway

作　　者：范丽 谢雪雁 袁媛[1] FAN Li;XIE Xueyan;YUAN Yuan(Department of Gynecology,Shenzhen Bao'an District Traditional Chinese Medicine Hospital,Shenzhen 518000,China)

机构地区：[1]深圳市宝安区中医院妇科,广东深圳518000

出　　处：《东南大学学报(医学版)》2025年第1期38-45,共8页Journal of Southeast University(Medical Science Edition)

基　　金：湖北省卫生健康委科研项目(WJ2023M169)。

摘　　要：目的:探讨梓醇联合罗格列酮调节高迁移率族蛋白B1(HMGB1)/Toll样受体4(TLR4)/核因子κB(NF-κB)信号通路对多囊卵巢综合征(PCOS)大鼠胰岛素抵抗及炎症的影响。方法:将72只大鼠随机分为PCOS组、梓醇组、罗格列酮组、梓醇+罗格列酮组、梓醇+罗格列酮+HMGB1激活剂(rr HMGB1)组、正常组。检测大鼠黄体生成素(LH)/促卵泡激素(FSH)、睾酮、雌二醇、空腹胰岛素、空腹血糖水平、胰岛素抵抗指数(HOMA-IR)及卵巢指数;HE染色检测卵巢组织病理;ELISA检测卵巢组织中肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β水平;Western blotting检测TLR4、HMGB1、p-NF-κB p65/NF-κB p65蛋白。结果:与PCOS组相比,梓醇组、罗格列酮组、梓醇+罗格列酮组卵巢滤泡囊肿减少,血清中LH/FSH、睾酮水平、空腹胰岛素、空腹血糖、HOMA-IR、卵巢指数、卵巢组织中TNF-α、IL-1β水平及TLR4、HMGB1、p-NF-κB p65/NF-κB p65蛋白降低,血清中雌二醇水平升高,且梓醇+罗格列酮组对应指标变化趋势最明显(P<0.05);rr HMGB1逆转了梓醇联合罗格列酮处理对PCOS大鼠的改善作用。结论:梓醇联合罗格列酮对PCOS大鼠胰岛素抵抗及炎症的改善作用可能与抑制HMGB1/TLR4/NF-κB通路的激活有关。Objective:To investigate the effects of catalpol combined with rosiglitazone on insulin resistance and inflammation in rats with polycystic ovary syndrome(PCOS)by regulating high mobility group protein B1(HMGB1)/Toll-like receptor 4(TLR4)/nuclear factorκB(NF-κB)pathway.Methods:72 rats were randomly divided into PCOS group,catalpol group,rosiglitazone group,catalpol+rosiglitazone group,catalpol+rosiglitazone+HMGB1 activator(rr HMGB1)group and normal group.Luteinizing hormone(LH)/follicle stimulating hormone(FSH),testosterone,estradiol,fasting insulin,fasting blood glucose levels,insulin resistance index(HOMA-IR),and ovarian index were measured in the rats.Pathological changes in ovarian tissue were detected using HE staining.Using ELISA,levels of tumor necrosis factor-α(TNF-α)and interleukin-1β(IL-1β)were detected in ovarian tissue.TLR4,HMGB1,and p-NF-κB p65/NF-κB p65 proteins were detected using Western blotting.Results:Compared with the PCOS group,the ovarian follicular cysts were reduced,the levels of LH/FSH,testosterone in serum,fasting insulin,fasting blood glucose,HOMA-IR,ovarian index,levels of TNF-α,IL-1β in ovarian tissue,and TLR4,HMGB1,p-NF-κB p65/NF-κB p65 proteins were decreased,the level of estradiol in serum was increased in the catalpol group,rosiglitazone group,and catalpol+rosiglitazone group,the changes of corresponding indicators in catalpol+rosiglitazone group was the most significant(P<0.05).Rr HMGB1 reversed the improvement effect of the combination of catalpol and rosiglitazone on PCOS rats.Conclusion:The effect of catalpol combined with rosiglitazone on insulin resistance and inflammation in PCOS rats may be related to inhibiting the activation of HMGB1/TLR4/NF-κB pathway.

关 键 词：梓醇 罗格列酮 多囊卵巢综合征 胰岛素抵抗 炎症 大鼠 

分 类 号：R-33[医药卫生] R711.75