β-甘露聚糖酶的性质、热稳定性改造及应用研究进展  

Research progress on properties,thermostability modification,and application ofβ-mannanase

作　　者：高小朋[1] 关福豪 郭超 王超 刘玉春 GAO Xiaopeng;GUAN Fuhao;GUO Chao;WANG Chao;LIU Yuchun(College of Life Science,Yan’an Univercity,Yan’an 716000,Shaanxi,China;Academy of National Food and Strategic Reserves Administration,Beijing 100037,China)

机构地区：[1]延安大学生命科学学院,陕西延安716000 [2]国家粮食和物资储备局科学研究院,北京100037

出　　处：《西安工程大学学报》2025年第1期33-43,共11页Journal of Xi’an Polytechnic University

基　　金：国家自然科学基金(22168041);中央级公益性科研院所基本科研业务费专项资金(ZX2416);陕西省重点科技创新团队建设项目(2023-CX-TD40)。

摘　　要：探讨β-甘露聚糖酶的性质、热稳定性及其在工业应用中的进展。β-甘露聚酶是一种能够水解β-1,4甘露糖苷键的内切糖苷水解酶,广泛应用于饲料、食品加工、纺织工业和生物燃料等领域。首先总结了β-甘露聚糖酶的基本特性,包括其在动植物和微生物中的分布,以及不同来源酶的最适反应条件和稳定性。随后,分析了影响β-甘露聚糖酶热稳定性的金属离子结合结构域、碳水化合物结合结构域、柔性区域和盐桥等结构因素,提出分子改造策略以提高其稳定性。通过结构改造提高β-甘露聚糖酶的热稳定性对其在工业中的应用至关重要,并讨论β-甘露聚糖酶在工业中的应用,特别是在饲料中作为益生元的应用,以及在食品加工、洗涤和纺织行业中的潜在用途,指出β-甘露聚糖酶的多功能性和对提高行业效率及可持续性的影响。最后对β-甘露聚糖酶新酶资源的挖掘、结构改造策略的优化,以及平衡酶的热稳定性和催化活性的未来研究方向进行了展望。We investigate the properties,thermal stability,and industrial applications ofβ-mannanase,an endoglucanase that hydrolyzesβ-1,4-mannosidic linkages.It is widely used in feed,food processing,textile industries,and biofuels.We first summarize the basic characteristics ofβ-mannanase,including its distribution in animals,plants,and microorganisms,as well as the optimal reaction conditions and stability of enzymes from different sources.Subsequently,we analyze the structural factors affecting the thermal stability ofβ-mannanase,such as metal ion-binding domains,carbohydrate-binding domains,flexible regions,and salt bridges,and propose molecular engineering strategies to enhance its stability,which is crucial for its industrial applications.We also discuss the industrial applications ofβ-mannanase,particularly as a prebiotic in feed,and its potential uses in food processing,detergent,and textile industries,highlighting its multifunctionality and impact on increasing industry efficiency and sustainability.Finally,we anticipate future research directions,including the exploration of new enzyme resources,optimization of structural modification strategies,and balancing the thermal stability and catalytic activity of enzymes.

关 键 词：Β-甘露聚糖酶 分子改造 热稳定性 酶学性质 理性设计 

分 类 号：Q55[生物学—生物化学]